- 中文名稱
Thioredoxin Reductase BioAssay Kit (TrxR) discontinued
- 英文名字
- Thioredoxin Reductase BioAssay Kit (TrxR) discontinued
- 供應(yīng)商
- USBiological
- 產(chǎn)品貨號
- T5010-17A
- 產(chǎn)品報(bào)價(jià)
- ￥1/100Tests
- 產(chǎn)品說明書
- 點(diǎn)擊查看
- 購買方式
- 銀行轉(zhuǎn)賬、電匯、支票、現(xiàn)金，在線支付寶及網(wǎng)銀支付，或直接與我們電話聯(lián)系400-6800-868
- 產(chǎn)品新聞
- 背景資料
- 產(chǎn)品描述
- Thioredoxin reductase (TrxR) (EC 1.8.1.9) is a ubiquitous enzyme which is involved in many cellular processes such as cell growth, p53 activity, and protection against oxidation stress, etc. The mammalian TrxR reduces thioredoxins as well as non-disulfide substrates such as selenite, lipoic acids, lipid hydroperoxides, and hydrogen peroxide. Thioredoxin Reductase BioAssay Kit provides a convenient colorimetric assay for detecting TrxR activity in various samples. In the assay TrxR catalyzes the reduction of 5, 5'-dithiobis (2-nitrobenzoic) acid (DTNB) with NADPH to 5-thio-2-nitrobenzoic acid (TNB 2-), which generate a strong yellow color (Lmax = 405 nm). Since in crude biological samples other enzymes, such as glutathione reductase and glutathione peroxidase, can also reduce DTNB, therefore, TrxR specific inhibitor is utilized to determine TrxR specific activity. Two assays are performed: the first measurement is of the total DTNB reduction by the sample, and the second one is the DTNB reduction by the sample in the presence of the TrxR specific inhibitor. The difference between the two results is the DTNB reduction by TrxR. Kit Components:1. TrxR Assay Buffer: 1x 25ml2. TNB Standard: 1x1 vial 3. DTNB: 1x1 vial 4. NADPH: 1x1 vial 5. TrxR Positive Control (~20mU) : 1x1 vial 6. TrxR Inhibitor: 1x1 vial Storage and Stability:Store kit at -20°C. Protect from light. Allow Assay Buffer to warm to room temperature before use. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- 產(chǎn)品特點(diǎn)
- 針對Thioredoxin Reductase BioAssay Kit (TrxR) discontinued該產(chǎn)品的特點(diǎn)優(yōu)勢，歡迎查閱官網(wǎng)提供的產(chǎn)品說明書。
- 保存建議
- 建議收到Thioredoxin Reductase BioAssay Kit (TrxR) discontinued產(chǎn)品后將其置于-20°C保存。
- 其他
- Usbiological公司是美國著名的抗體和生化試劑供應(yīng)商，生產(chǎn)世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細(xì)胞術(shù)等多種檢測方法。Usbiological公司現(xiàn)已擁有超過50,19234種抗體、抗原和生化產(chǎn)品，為科研用戶提供了諸多超值選擇。
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- 注意
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該頁面的中文產(chǎn)品信息的翻譯，僅供參考。準(zhǔn)確的產(chǎn)品信息請以廠家的英文說明書為準(zhǔn)。下單前，請瀏覽說明書確認(rèn)。
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