- 中文名稱(chēng)
Thyroid Stimulating Hormone, Human, Low Range (TSH) BioAssay ELISA Kit
- 英文名字
- Thyroid Stimulating Hormone, Human, Low Range (TSH) BioAssay ELISA Kit
- 供應(yīng)商
- USBiological
- 產(chǎn)品貨號(hào)
- T5400-01
- 產(chǎn)品報(bào)價(jià)
- ￥1/96Tests
- 產(chǎn)品說(shuō)明書(shū)
- 點(diǎn)擊查看
- 購(gòu)買(mǎi)方式
- 銀行轉(zhuǎn)賬、電匯、支票、現(xiàn)金，在線支付寶及網(wǎng)銀支付，或直接與我們電話聯(lián)系400-6800-868
- 產(chǎn)品新聞
- 背景資料
- 產(chǎn)品描述
- This ultra-sensitive TSH enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for TSH. Standards or samples are then added to the microtiter plate wells and TSH if present, will bind to the antibody precoated on the wells. In order to quantitate the amount of TSH present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for TSH are added to each well to “sandwich” the TSH immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, a TMB (3,3', 5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain TSH and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm.In order to measure the concentration of TSH in the sample this Ultra-sensitive Human TSH ELISA Kit includes a set of calibration standards (6 standards). The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density (O.D.) versus TSH concentration (uIU/ml). The concentration of TSH in the samples is then determined by comparing the O.D. of the samples to the standard curve.Sensitivity: <0.05uIU/mlRange: 0.05-12uIU/mlKit Components:1. Microtiter Plate, 1x96 wells2. Conjugate, 1x12ml3. Standard, 12uIU/ml, 1x1 vial4. Standard, 6 uIU/ml, 1x1 vial5. Standard, 2ulU/ml, 1x1 vial6. Standard, 0.8ulU/ml, 1x1 vial7. Standard, 0.2ulU/ml, 1x11 vial8. Standard, 0ulU/ml, 1x1 vial9. Substrate A, 1x10ml10. Substrate B, 1x10ml11. Stop Solution, 1x7ml, 2N Sulphuric Acid (H2SO4). Storage and Stability:Store components at -20°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.?
- 產(chǎn)品特點(diǎn)
- 針對(duì)Thyroid Stimulating Hormone, Human, Low Range (TSH) BioAssay ELISA Kit該產(chǎn)品的特點(diǎn)優(yōu)勢(shì)，歡迎查閱官網(wǎng)提供的產(chǎn)品說(shuō)明書(shū)。
- 保存建議
- 建議收到Thyroid Stimulating Hormone, Human, Low Range (TSH) BioAssay ELISA Kit產(chǎn)品后將其置于-20°C保存。
- 其他
- Usbiological公司是美國(guó)著名的抗體和生化試劑供應(yīng)商，生產(chǎn)世界上種類(lèi)最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細(xì)胞術(shù)等多種檢測(cè)方法。Usbiological公司現(xiàn)已擁有超過(guò)50,19234種抗體、抗原和生化產(chǎn)品，為科研用戶提供了諸多超值選擇。
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- 注意
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該頁(yè)面的中文產(chǎn)品信息的翻譯，僅供參考。準(zhǔn)確的產(chǎn)品信息請(qǐng)以廠家的英文說(shuō)明書(shū)為準(zhǔn)。下單前，請(qǐng)瀏覽說(shuō)明書(shū)確認(rèn)。
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