- 中文名稱
Tissue Inhibitor of Metalloproteinases 1 (TIMP1) BioAssay ELISA Kit (Erythroid Potentiating Activity, EPA, Collagenase Inhibitor, CI)
- 英文名字
- Tissue Inhibitor of Metalloproteinases 1 (TIMP1) BioAssay ELISA Kit (Erythroid Potentiating Activity, EPA, Collagenase Inhibitor, CI)
- 供應(yīng)商
- USBiological
- 產(chǎn)品貨號
- T5580-50
- 產(chǎn)品報(bào)價(jià)
- ￥1/1Kit
- 產(chǎn)品說明書
- 點(diǎn)擊查看
- 購買方式
- 銀行轉(zhuǎn)賬、電匯、支票、現(xiàn)金，在線支付寶及網(wǎng)銀支付，或直接與我們電話聯(lián)系400-6800-868
- 產(chǎn)品新聞
- 背景資料
- 產(chǎn)品描述
- Tissue inhibitor of metalloproteinases-1 (TIMP-1) has been demonstrated in numerous tissues and body fluids as one of the important regulatory factors in connective tissue metabolism. TIMP-1 is a glycoprotein (M.W. 32kD) that inhibits collagenases, gelatinases and stromelysin activities, and that is identical with collagenase inhibitor of ? 1-anticollagenase. TIMP-1 may be quantitated using this sensitive immunoassay. The TIMP-1 immunoassay kit is a solid phase enzyme immunoassay (EIA) based on one-step sandwich immunoreaction. Upon addition of TIMP-1 containing specimen, the result is a TIMP-1 molecule being sandwiched between the solid phase and enzyme labeled antibodies. After removing unbound enzyme-labeled antibody, the well is then incubated with enzyme substrate and o-phenylene-diamine (OPD), resulting in the development of color. The activity of peroxidase enzyme is proportional to the amount of antigen, so that TIMP-1 concentration in specimens can be determined from a standard curve.The TIMP-1 immunoassay kit is useful for the determination of TIMP-1 levels in fresh human serum and other body fluids. Antibodies in the kit are also reactive with bovine TIMP-1. Contents of this kit are sufficient for assay of 96 samples, including standard curve. Testing of samples in duplicate or triplicate is strongly recommended. Analytical Sensitivity and Detection Limits Sensitivity: 1.2ng/ml Range of Detection: 1.2ng/ml to 49ng/ml Typical values: TIMP-1 values of: Serum: 183 +/-30ng/ml Platelet poor plasma 68 +/-13ng/ml Amniotic fluid 1780 +/-969 CSF 60 +/-13 Tear fluid 418 +/-145 Mixed saliva 316 +/-166 Urine 7 +/-8 Kit Components:1. Anti-TIMP-1 Coated Microplate–One (1) plate containing 96 coated wells. Microplate lid included. 2.Antibody-HRP Conjugate–One (1) 1.2ml bottle.3. Buffer (Lyophilized) – One (1) bottle bovine serum albumin in sodium phosphate buffer, sufficient for 20ml.4. Color Reagent-1 (Lyophilized) – Three (3) bottles o-Phenylenediamine (OPD), sufficient for 5ml.5. Color Reagent-2 – One (1) 20ml bottle containing 0.02% Hydrogen peroxide.6. Stop Solution – One (1) 15ml bottle containing 2 N Sulfuric acid.7. TIMP-1 Standard (Lyophilized)–One (1) bottle, sufficient to make 0.5ml TIMP-1.8. Buffered Detergent – One (1) 1ml bottle Tween-20 in sodium phosphate buffer.Storage and Stability: Store components at 4°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.?
- 產(chǎn)品特點(diǎn)
- 針對Tissue Inhibitor of Metalloproteinases 1 (TIMP1) BioAssay ELISA Kit (Erythroid Potentiating Activity, EPA, Collagenase Inhibitor, CI)該產(chǎn)品的特點(diǎn)優(yōu)勢，歡迎查閱官網(wǎng)提供的產(chǎn)品說明書。
- 保存建議
- 建議收到Tissue Inhibitor of Metalloproteinases 1 (TIMP1) BioAssay ELISA Kit (Erythroid Potentiating Activity, EPA, Collagenase Inhibitor, CI)產(chǎn)品后將其置于4°C保存。
- 其他
- Usbiological公司是美國著名的抗體和生化試劑供應(yīng)商，生產(chǎn)世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細(xì)胞術(shù)等多種檢測方法。Usbiological公司現(xiàn)已擁有超過50,19234種抗體、抗原和生化產(chǎn)品，為科研用戶提供了諸多超值選擇。
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- 注意
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該頁面的中文產(chǎn)品信息的翻譯，僅供參考。準(zhǔn)確的產(chǎn)品信息請以廠家的英文說明書為準(zhǔn)。下單前，請瀏覽說明書確認(rèn)。
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