- 中文名稱
Tissue Inhibitor of Metalloproteinases 2 (TIMP2) BioAssay ELISA Kit
- 英文名字
- Tissue Inhibitor of Metalloproteinases 2 (TIMP2) BioAssay ELISA Kit
- 供應(yīng)商
- USBiological
- 產(chǎn)品貨號(hào)
- T5585-75
- 產(chǎn)品報(bào)價(jià)
- ￥1/1Kit
- 產(chǎn)品說(shuō)明書
- 點(diǎn)擊查看
- 購(gòu)買方式
- 銀行轉(zhuǎn)賬、電匯、支票、現(xiàn)金，在線支付寶及網(wǎng)銀支付，或直接與我們電話聯(lián)系400-6800-868
- 產(chǎn)品新聞
- 背景資料
- 產(chǎn)品描述
- The TIMP-2 immunoassay kit utilizes antibodies immobilized on a bead matrix, in combination with enzyme-labeled antibodies, directed against different antigenic sites on the same TIMP-2 molecule. Upon addition of a TIMP-2-containing specimen, the result is a TIMP-2 molecule being sandwiched between the solid phase and enzyme labeled antibodies. After removing unbound enzyme-labeled antibody, the bead containing the sandwich is incubated with enzyme substrate and TMBZ, resulting in the development of color. The activity of peroxidase enzyme is proportional to the amount of antigen, TIMP-2, so that TIMP-2 concentration in specimens can be determined from a standard curve. The TIMP-2 immunoassay kit is useful for the determination of TIMP-2 levels in fresh serum and conditioned medium of cells. This system does not recognize TIMP-2 complexed with proMMP-2. While the immunoreactivities of TIMP-2 complexed with active MMP-1, MMP-2 or MMP-9 are increased by approximately 300%, 450%, and 300%, respectively, compared with that of the free form of TIMP-2, no change is observed when TIMP-2 is complexed with active MMP-3. Contents of this kit are sufficient for assay of 100 samples, including standard curve. Testing of samples in duplicate or triplicate is strongly recommended. This kit is intended for research use only; not for diagnostic or therapeutic applications. Sensitivity: 20ng/ml Range of Detection: 20ng/ml – 320ng/mlKit Components:1. Anti-TIMP-2 Coated Beads–One (1) bottle containing 100 anti-TIMP-2 coated polystyrene beads.2. Concentrated Enzyme Labeled Antibody Solution–One (1) 1ml bottle containing TIMP-2 antibody.3. Color Reagent– One (1) 1ml bottle 3,3',5,5'-Tetramethylbenzidine (TMBZ)4. Buffer for Color Reagent: 40ml Acetate buffer, pH 5.5.5. Substrate Solution–One (1) 15ml bottle 0.02% Hydrogen Peroxide.6. Stop Solution–Two (2) bottles, sufficient to make 100ml 1.3 N Sulfuric acid per bottle.7. Buffer Reagent (Lyophilized)–One (1) 50ml bottle BSA in sodium phosphate buffer.8. TIMP-2 Standard (Lyophilized)–One (1) bottle, sufficient to make 1ml Human TIMP-2 Standard.9. Concentrated Wash Solution–Two bottles, sufficient to make 1L Sodium phosphate buffer, pH 7.0, per bottle.Storage and Stability:Store components at 4°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.?
- 產(chǎn)品特點(diǎn)
- 針對(duì)Tissue Inhibitor of Metalloproteinases 2 (TIMP2) BioAssay ELISA Kit該產(chǎn)品的特點(diǎn)優(yōu)勢(shì)，歡迎查閱官網(wǎng)提供的產(chǎn)品說(shuō)明書。
- 保存建議
- 建議收到Tissue Inhibitor of Metalloproteinases 2 (TIMP2) BioAssay ELISA Kit產(chǎn)品后將其置于4°C保存。
- 其他
- Usbiological公司是美國(guó)著名的抗體和生化試劑供應(yīng)商，生產(chǎn)世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細(xì)胞術(shù)等多種檢測(cè)方法。Usbiological公司現(xiàn)已擁有超過(guò)50,19234種抗體、抗原和生化產(chǎn)品，為科研用戶提供了諸多超值選擇。
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- 注意
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該頁(yè)面的中文產(chǎn)品信息的翻譯，僅供參考。準(zhǔn)確的產(chǎn)品信息請(qǐng)以廠家的英文說(shuō)明書為準(zhǔn)。下單前，請(qǐng)瀏覽說(shuō)明書確認(rèn)。
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