- 中文名稱
Triiodothyronine Uptake, Human, BioAssay ELISA Kit (T3)
- 英文名字
- Triiodothyronine Uptake, Human, BioAssay ELISA Kit (T3)
- 供應商
- USBiological
- 產品貨號
- T8425-04
- 產品報價
- ￥1/96Tests
- 產品說明書
- 點擊查看
- 購買方式
- 銀行轉賬、電匯、支票、現(xiàn)金，在線支付寶及網銀支付，或直接與我們電話聯(lián)系400-6800-868
- 產品新聞
- 背景資料
- 產品描述
- Triiodothyronine Uptake, Human, BioAssay ELISA Kit (T3) permits the measurement of the total amount of binding sites available for the thyroid hormones in human serum or plasma by a microplate enzyme immunoassay.Triiodothyronine also known as T3, is a thyroid hormone. It affects almost every physiological process in the body, including growth and development, metabolism, body temperature, and heart rate. Together with thyroxine (T4), T3 exerts a potent influence on the basal metabolic rate. T3 is synthesized from the tyrosine residues of thyroglobin and is found in the serum of normal individuals. Although T3 has a lower serum concentration than T4, it is 5 to 10 times more active.This microplate enzyme immunoassay methodology provides the technician with optimum sensitivity while requiring few technical manipulations. In this method, serum reference, patient specimen, or control is first added to a microplate well. Enzyme-T3 conjugate and thyroxine (T4) are added, and then the reactants are mixed. The endogenous binding proteins of the sample react with the thyroxine, but not with the enzyme conjugate. This leads to a higher binding of the enzyme conjugate to the antibody combining sites, reactive for triiodothyronine and thyroxine, immobilized on the well as the binding capacity of the specimen increases. After the completion of the required incubation period, the antibody bound enzyme-triiodothyronine conjugate is separated from the unbound enzyme-triiodothyronine conjugate by aspiration or decantation. The activity of the enzyme present on the surface of the well is quantitated by reaction with a suitable substrate to produce color. The employment of several serum references of known unsaturated thyroid hormone binding capacity permits construction of a graph of absorbance and concentration. From comparison to the dose response curve, an unknown specimen's absorbance can be correlated with thyroid hormone binding capacity.Kit Components: T8425-04A: Microtiter Plate, 1x96 wellsT8425-04B: Calibrator, 18%U, 1x1mlT8425-04C:. Calibrator, 24.5%U, 1x1mlT8425-04D: Calibrator, 34%U, 1x1mlT8425-04E: Calibrator, 48%U, 1x1mlT8425-04F. T3 (HRP), 1x1.5mlT8425-04G: HRP Buffer, 1x13mlT8425-04H: Substrate A (TMB), 1x7mlT8425-04J: Substrate B (H2O2), 1x7mlT8425-04K: Wash Buffer, 50X, 1x20mlT8425-04L: Stop Solution (1N HCl), 1x8ml Storage and Stability:Store all components at 4°C. Stable for 6 months. Opened reagents are stable for 60 days at 4°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- 產品特點
- 針對Triiodothyronine Uptake, Human, BioAssay ELISA Kit (T3)該產品的特點優(yōu)勢，歡迎查閱官網提供的產品說明書。
- 保存建議
- 建議收到Triiodothyronine Uptake, Human, BioAssay ELISA Kit (T3)產品后將其置于4°C保存。
- 其他
- Usbiological公司是美國著名的抗體和生化試劑供應商，生產世界上種類最多的抗體，用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細胞術等多種檢測方法。Usbiological公司現(xiàn)已擁有超過50,19234種抗體、抗原和生化產品，為科研用戶提供了諸多超值選擇。
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- 注意
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該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。
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