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中文名稱

Varicella Zoster Virus, Human, IgG, BioAssay ELISA Kit (VZV)

英文名字
Varicella Zoster Virus, Human, IgG, BioAssay ELISA Kit (VZV)
供應(yīng)商
USBiological
產(chǎn)品貨號(hào)
V2100-29
產(chǎn)品報(bào)價(jià)
¥1/1Kit
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產(chǎn)品描述
The United States Biological Varicella Zoster Virus, Human, IgG, BioAssay ELISA Kit is intended for the detection and quantitative determination of IgG antibody to VZV in sample serum. Individual serum specimens may be used for the determination of immune status. Paired serum, acute and convalescent, may be used to demonstrate seroconversion or a significant rise in antibody level.Varicella, more commonly known as chickenpox, and herpes zoster are known clinical manifestations of infection with varicella-zoster virus (VZV).Chicken pox, the clinical syndrome usually produced as a result of the primary infection with VZV, is a highly contagious disease characterized by widely spread vesicular eruptions and fever. The disease is endemic in the U.S. and most commonly affects children from five to eight years of age, although adults and younger children, including infants, may develop chickenpox. Every two to five years, usually in the winter or spring, the number of cases increases to epidemic levels. VZV infection during early pregnancy has been implicated in congenital anomalies in rare cases. When infection occurs at term, life-threatening infections can occur in the neonate.Herpes zoster is mainly a disease of adults, with most cases appearing in samples fifty years or older. Evidence suggests that this manifestation of VZV infections results from a reactivation of virus which has remained latent in the sensory spinal ganglia after the primary infection rather than a reintroduction of the virus into the host. Fever and painful localized vesicular eruptions of the skin along the distribution of the involved nerves are the most common clinical symptoms of the condition. Zoster lesions can be mistaken for the similar lesions produced by herpes simplex virus in which recurrences are common. However, recurrences of herpes zoster are extremely rare.Determination of the immune status of high risk individuals who are exposed to VZV, the screening for potential donors of Varicella-zoster immunoglobulin, and the diagnosis of VZV infected individuals (both pre- and postnatal) is usually accomplished by serological testing. The various methods for the detection of VZV antibodies in a sample's serum include indirect immunofluorescence, neutralization, complement fixation and fluorescent antibody to membrane antigen (FAMA). FAMA is generally considered the most sensitive and specific of the methods, yet requires the use of cell culture which is cumbersome to perform. Clinical and correlation studies performed by Shehab and Brunell indicate that the ELISA methodology may be as sensitive and perhaps more specific than the FAMA assay. The sensitivity, specificity, and reproducibility of ELISAs are comparable to other serological tests for antibody, such as immunofluorescence, complement fixation, hemagglutination and radioimmunoassays.The United States Biological Varicella Zoster Virus, Human, IgG, BioAssay ELISA Kit provides all the necessary reagents for the rapid quantitative determination of VZV IgG antibody in sample serum.Kit Components:1. Microtiter Plate, Varicella-zoster virus antigen (inactivated): 1 X 96 wells2. Serum Diluent: 1X30ml3. Calibrator (sample serum or defibrinated plasma): 1X400ul4. Positive Control: 1X400ul5. Negative Control: 1X400ul6. IgG (HRP) Goat x human: 1X16ml7. Tetramethylbenzidine (TMB): 1x15ml 8. Wash Buffer, 20X: 1x50ml9. Stop Solution: 1x15ml (1N H2SO4)Storage and Stability:Store all components at 4°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
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Usbiological公司是美國著名的抗體和生化試劑供應(yīng)商,生產(chǎn)世界上種類最多的抗體,用于Western Blot、免疫沉淀、免疫熒光、免疫組化和流式細(xì)胞術(shù)等多種檢測方法。Usbiological公司現(xiàn)已擁有超過50,19234種抗體、抗原和生化產(chǎn)品,為科研用戶提供了諸多超值選擇。
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