中文名稱

【干冰】Mouse Brain, adult, Whole Cell Lysate - W10-000-T004

英文名字

【干冰】Mouse Brain, adult, Whole Cell Lysate - W10-000-T004

供應(yīng)商

Rockland

產(chǎn)品貨號(hào)

W10-000-T004

產(chǎn)品報(bào)價(jià)

￥詢價(jià)/500ug

產(chǎn)品說(shuō)明書(shū)

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購(gòu)買(mǎi)方式

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產(chǎn)品新聞

背景資料

Ready-to-use whole cell lysates produced by Rockland Immunochemicals are derived from cell lines or tissues using highly refined extraction protocols to ensure exceptionally high quality, protein integrity and lot-to-lot reproducibility.? All extracts are tested by SDS-PAGE using 4-20% gradient gels and immunoblot analysis using antibodies to key cell signaling components to confirm the presence of both high molecular weight and low molecular weight proteins.

產(chǎn)品描述

Ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been added). Heat lysate to 95° C for 5 minutes and rapidly cool.? If dissociating conditions are desired, add reducing agent prior to heating.? The recommended loading volume per lane is 10-20 μl depending on the size format of your gel.

產(chǎn)品形式

Liquid (sterile filtered)

保存建議

Store vial at -70° C or COLDER. For extended storage, aliquot contents to minimize freeze/thaw cycles.

其他

Rockland成立于1962年，是一家老牌抗體原產(chǎn)企業(yè)，其位于美國(guó)費(fèi)城郊區(qū)的農(nóng)場(chǎng)保有近20種實(shí)驗(yàn)動(dòng)物。Rockland公司為生命科學(xué)領(lǐng)域提供豐富的、高質(zhì)量的抗體，包括載脂蛋白、細(xì)胞周期/凋亡、細(xì)胞因子、細(xì)胞信號(hào)蛋白、酶類、細(xì)胞外基質(zhì)、轉(zhuǎn)錄因子(NFkB)和泛素（Ubiquitin）等識(shí)別抗體，應(yīng)用范圍包括Western blot、免疫組化、免疫熒光和ELISA等。另外，Rockland也生產(chǎn)特別齊全的二抗，包括純化、生物素、HRP/ALP、熒光標(biāo)記二抗，應(yīng)用十分廣泛。

注意

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