中文名稱

Secretin Human

英文名字

Secretin Human

供應(yīng)商

Aviva Systems Biology

產(chǎn)品貨號

aviva-GWB-35EB92-1MG

產(chǎn)品報價

￥詢價/1mg

產(chǎn)品說明書

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購買方式

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產(chǎn)品新聞

背景資料

Product Name	Estradiol ELISA**
Sample Type	Serum
# of Samples	1x96 well
Intended Use	The GenWay, Inc. Estradiol (E2) ELISA Kit is intended for the quantitative determination of Estradiol (E2) concentration in human serum.
Principle of the test	The GenWay Biotech, Inc E2 ELISA kit is based on the principle of Delayed competitive binding assay between E2 in the test specimen and E2 enzyme conjugate for a constant amount of anti-Estradiol monoclonal antibody epitopes (Biotin reagent). In the incubation, anti-E2 antibody biotin reagent, E2 standards, controls, and samples are incubated for 45 minutes at room temperature (RT), then E2 enzyme conjugate is added on the top of the reaction mixture and incubation continues for 45 minutes more. During the incubation, a fixed amount of HRP-labeled E2 competes with the endogenous E2 in the standard, sample, or quality control serum for a fixed number of binding sites of the specific E2 antibody. E2 peroxidase conjugate immunologically bound to the well progressively decreases as the concentration of E2 in the specimen increases. Unbound of anti-Estradiol Biotin Reagent and E2 peroxidase conjugate is then removed and the wells are washed. Next, a solution of TMB Reagent is added and incubated at room temperature for 20 minutes, resulting in the development of blue color. The color development is stopped with the addition of stop solution, and the absorbance is measured spectrophotometrically at 450 nm. A standard curve is obtained by plotting the concentration of the standard versus the absorbance
Storage and Stability	2-8C.
References	Horm Behav 2013 Jan;63(1):144-57. doi: 10.1016/j.yhbeh.2012.09.011. Epub 2012 Oct 12. 17β-Estradiol, but not estrone, increases the survival and activation of new neurons in the hippocampus in response to spatial memory in adult female rats. McClure RE, Barha CK, Galea LA Department of Psychology, Graduate Program in Neuroscience, Brain Research Centre, University of British Columbia, Vancouver, BC, Canada.

序列

該Secretin Human的序列請參考該產(chǎn)品的說明書

來源宿主

該Secretin Human的來源宿主請參考該產(chǎn)品的說明書

溶解建議

該Secretin Human的溶解建議請參考該產(chǎn)品的說明書

保存建議

該Secretin Human的保存建議請參考該產(chǎn)品的說明書

其他

Aviva Systems Biology總部位于加利福尼亞州圣迭戈，在中國北京設(shè)有辦公室，專注于為研究需求提供多克隆和單克隆抗體、ELISA試劑盒、蛋白質(zhì)和定制服務(wù)。Aviva Systems Biology生產(chǎn)了24,000種經(jīng)過驗證的多克隆抗體，并提供近20,000種ELISA試劑盒，定制實驗室服務(wù)包括蛋白表達和純化、抗體開發(fā)，以及ELISA的開發(fā)、驗證和生產(chǎn)。Aviva Systems Biology為與獨特物種和靶標(biāo)相關(guān)的研究提供獨特工具，研究領(lǐng)域包括轉(zhuǎn)錄因子、癌癥、心血管、細胞生物學(xué)、DNA損傷和修復(fù)、表觀遺傳學(xué)、信號轉(zhuǎn)導(dǎo)、細胞分化、干細胞生物學(xué)等等。

注意

該頁面的中文產(chǎn)品信息的翻譯，僅供參考。準(zhǔn)確的產(chǎn)品信息請以廠家的英文說明書為準(zhǔn)。下單前，請瀏覽說明書確認。

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