中文名稱

Adenosine Deaminase（ADA）Calibrator（2 mL Vial）（阿登西汀脫氨酶（ADA）Calibrator（2 mL Vial））

英文名字

Adenosine Deaminase (ADA) Calibrator (2 mL Vial)

供應(yīng)商

Aviva Systems Biology

產(chǎn)品貨號

aviva-GWB-BQK2A7-1U

產(chǎn)品報價

￥詢價/1 x 1 mL

產(chǎn)品說明書

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購買方式

銀行轉(zhuǎn)賬、電匯、支票、現(xiàn)金，在線支付寶及網(wǎng)銀支付，或直接與我們電話聯(lián)系。本公司所有產(chǎn)品僅供科研使用，不用于臨床診斷。

產(chǎn)品新聞

背景資料

該Adenosine Deaminase (ADA) Calibrator (2 mL Vial)的背景資料請參考該產(chǎn)品的說明書

產(chǎn)品描述

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Product Name	Adenovirus IgM - ELISA.
# of Samples	1 x 96 Assays.
Intended Use	The Adenovirus IgM ELISA is intended for the qualitative determination of IgM class antibodies against Adenovirus in human serum or plasma (citrate).
Introduction	Adenoviruses are double-stranded DNA viruses of about 70-90 nm lacking an envelope. The capsid contains 252 capsomeres and shows icosahedral symmetry. The capsomeres consist of hexons, pentons and fiberprotein trimers which are responsible for the induction of group- and type-specific antibodies. For the first time adenoviruses were isolated in 1953 from tonsils and adenoid tissue by Rowe. More than 80 adenoviruses are known at present. 47 out of them are pathogenic for men. They cause several diseases of different organic systems, mainly eyes, pharynx, respiratory and gastrointestinal system. Adenovirus infections are common and frequent. Most infections appear during childhood. They pass of latently so that the virus can still be detected in tonsils after two years. It is excrete via saliva and faeces. Gate to body are mouth, nasal pharynx and conjunctiva of the eye. Most infections pass off without symptoms. Around 5 % of all coughs and sneezes of children are caused by adenoviruses. Epidemics may occur in populations crowded together, for example acute respiratory disease in military groups, pharyngoconjunctival fever in swimming pools, and epidemic keratoconjunctivitis in medical facilities. Infection of hospitals and swimming pools gratify special demands on hygienics. The presence of virus resp. infection may be identified by Cell culture: cytopathogenic effect (CPE) PCR Serology: complement fixation (CF), neutralization (N) and hemagglutination-inhibition (HAI); Detection of antibodies and the hexon antigen by ELISA.
Principles of the assay	The qualitative immunoenzymatic determination of IgM-class antibodies against Adenovirus is based on the ELISA (Enzyme-linked Immunosorbent Assay) technique. Microtiter strip wells are precoated with Adenovirus antigens to bind corresponding antibodies of the specimen. After washing the wells to remove all unbound sample material horseradish peroxidase (HRP) labelled anti-human IgM conjugate is added. This conjugate binds to the captured Adenovirus-specific antibodies. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of Adenovirus specific IgM antibodies in the specimen. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450 nm is read using an ELISA microwell plate reader.
Storage and Stability	The reagents are stable up to the expiry date stated on the label when stored at 2-8C.
References	Adrian,T.,J.Sassinek,R.Wiegand:Genome type analysis of 480 isolates of adenovirus type 1,2 and 5.Arch Virol.112 (1990) 235-248Donelli,G.,F.Superti,A.Tinari et al.: Viral childhood diarrhoe in Rome: a diagnostic and epidemiological study.Microbiologica 16 (1993) 215-225Hierholzer,J.C.: Adenovirus in the immunocompromised host. Clin.Microbiol.Rev.5 (1992) 262-274Schmitz,H.,R.Wiegand,W.Heinrich: Worlwide epidemiology of human adenovirus infectionsw.Am.J.Epid.117 (1983) 455-466Wiegand,R.:Adenovirus.In:Brandis,H.,W.Köhler,H.J.Eggers,G.Pulverer (ed.):Med. Mikrobiologie 1994.Gustav Fischer Verlag Stuttgart,Jena,New York (1994) 767-769

產(chǎn)品形式

該Adenosine Deaminase (ADA) Calibrator (2 mL Vial)的產(chǎn)品形式請參考該產(chǎn)品的說明書

保存建議

該Adenosine Deaminase (ADA) Calibrator (2 mL Vial)的保存建議請參考該產(chǎn)品的說明書

其他

Aviva Systems Biology總部位于加利福尼亞州圣迭戈，在中國北京設(shè)有辦公室，專注于為研究需求提供多克隆和單克隆抗體、ELISA試劑盒、蛋白質(zhì)和定制服務(wù)。Aviva Systems Biology生產(chǎn)了24,000種經(jīng)過驗證的多克隆抗體，并提供近20,000種ELISA試劑盒，定制實驗室服務(wù)包括蛋白表達和純化、抗體開發(fā)，以及ELISA的開發(fā)、驗證和生產(chǎn)。Aviva Systems Biology為與獨特物種和靶標相關(guān)的研究提供獨特工具，研究領(lǐng)域包括轉(zhuǎn)錄因子、癌癥、心血管、細胞生物學、DNA損傷和修復(fù)、表觀遺傳學、信號轉(zhuǎn)導(dǎo)、細胞分化、干細胞生物學等等。

注意

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