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中文名稱(chēng)

腎(MDBK)細(xì)胞裂解物

英文名字
Kidney (MDBK) Cell Lysate
供應(yīng)商
Aviva Systems Biology
產(chǎn)品貨號(hào)
aviva-OCEG01196-100UL
產(chǎn)品報(bào)價(jià)
¥詢(xún)價(jià)/100ul
產(chǎn)品說(shuō)明書(shū)
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產(chǎn)品新聞
背景資料
該Kidney (MDBK) Cell Lysate的背景資料請(qǐng)參考該產(chǎn)品的說(shuō)明書(shū)
產(chǎn)品描述
Total cell Iysates are useful as both positive and negative controls in immunoblotting. A431, a human epidermoid carcinoma cell line, and the EGF-stimulated A431 Iysates are used as negative and positive controls, respectively, when studying the phosphorylation cascade initiated by ligand binding to receptor tyrosine kinases. Both A431 and normal human fibroblasts are valuable positive controls for antibodies to proteins expressed in these cells. The mouse fibroblast cell line 3T3 and its RSV-transformed counterpart serve as negative and positive controls, respectively, for antibodies to proteins that are phosphorylated as a result of transformation by RSV (Rous sarcoma virus). Madin-Darby bovine kidney (MDBK) cell Iysate displays proteins expressed in bovine kidney and PC12 (rat phaeochromocytoma) cell Iysate can be expected to mirror the protein expression of cells and tissues of neuroectodermal origin. Likewise, Jurkat cells express the proteins unique to human T-cells. Rat brain is a widely used positive control for those proteins that are exclusively expressed in brain. At right is an example of the use of positive and negative control Iysates in western blotting. The blot of 3T3 cell Iysate (lane 1) and RSV-transformed 3T3 (lane 2) was probed with anti-phosphotyrosine peroxidase conjugate to reveal those proteins that are tyrosine-phosphorylated in response to transformation by
產(chǎn)品形式
Liquid Solution in Laemmli electrophoresis sample buffer
保存建議
該Kidney (MDBK) Cell Lysate的保存建議請(qǐng)參考該產(chǎn)品的說(shuō)明書(shū)
其他
Aviva Systems Biology總部位于加利福尼亞州圣迭戈,在中國(guó)北京設(shè)有辦公室,專(zhuān)注于為研究需求提供多克隆和單克隆抗體、ELISA試劑盒、蛋白質(zhì)和定制服務(wù)。Aviva Systems Biology生產(chǎn)了24,000種經(jīng)過(guò)驗(yàn)證的多克隆抗體,并提供近20,000種ELISA試劑盒,定制實(shí)驗(yàn)室服務(wù)包括蛋白表達(dá)和純化、抗體開(kāi)發(fā),以及ELISA的開(kāi)發(fā)、驗(yàn)證和生產(chǎn)。Aviva Systems Biology為與獨(dú)特物種和靶標(biāo)相關(guān)的研究提供獨(dú)特工具,研究領(lǐng)域包括轉(zhuǎn)錄因子、癌癥、心血管、細(xì)胞生物學(xué)、DNA損傷和修復(fù)、表觀遺傳學(xué)、信號(hào)轉(zhuǎn)導(dǎo)、細(xì)胞分化、干細(xì)胞生物學(xué)等等。
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