- 中文名稱
宿主細(xì)胞蛋白（大腸桿菌）ELISA試劑盒
- 英文名字
- Host Cell Proteins (E.coli) ELISA kit
- 供應(yīng)商
- Bertin Bioreagent
- 產(chǎn)品貨號(hào)
- A05034
- 產(chǎn)品報(bào)價(jià)
- ￥詢價(jià)/96wells
- 產(chǎn)品說明書
- 點(diǎn)擊查看
- 購(gòu)買方式
- 90%產(chǎn)品中國(guó)有現(xiàn)貨庫(kù)存。銀行轉(zhuǎn)賬、電匯、支票、現(xiàn)金，在線支付寶及網(wǎng)銀支付，或直接與我們電話聯(lián)系400-6800-868
- 產(chǎn)品新聞
- 背景資料
- Enzyme ImmunoAssay (EIA) is a technique to detect and quantify antigens (proteins, hormones…) or antibodies in samples. It relies on the ability of an antibody to bind a specific antigen. Either the antibody or the antigen is labelled with an enzyme whose substrate is a chromogen or a fluorogen converted in a measurable product (color or fluorescence).|Enzyme-linked Immunosorbent Assay (ELISA) is a type of EIA using a solid phase (ex: microtiter plate) coated with an antigen immobilizing the molecule to detect. Over the time, scientists have extended the term ELISA to EIAs using an antibody coating the solid phase. That explains why our EIA kits using coated antibodies are also called ELISA kits.|Impurity assessment is a key step during the drug development production of recombinant proteins, including therapeutic proteins. Specific impurities coming from the cells mediating the protein expression, known as Host Cell Proteins (HCP) are generated and need to be removed. This kit is intended for use in assessing relative quantities of E. coli HCP in manufactured or research bioproducts.|Polyclonal antibodies used in this kit have been generated against several strains of E.coli and specifically selected for their recognition of a large spectrum of E. coli proteins. Thus, this kit can be considered as generic and allows a relative-quantitative determination of E. coli HCP in many types of samples, such as samples issued from the purification process (HCP clearance), process control, quality control, or product release.|Using this kit, HCP concentration is measured in ng/ml (HCP equivalent is extrapolated from a standard curve). Conventionally, the HCP content in a product will finally be expressed in ng/mg, where ng represents HCP mass and mg represents the product mass. Note that, contrary to the concentration measurement of the product, the HCP signal is only reflective of antibody binding and does not strictly reflect the mass of HCP.|This kit has been successfully validated for recovery and precision using reconstituted HCP samples and tested against different final products. Given the diversity of final products, all potential matrix effects cannot be known and it is recommended that you test the suitability of the kit for use with your own HCP samples in your laboratory.|This kit should be used as one part of your complete HCP analysis.
- 產(chǎn)品描述
- 酶免疫測(cè)定（EIA）是一種檢測(cè)和定量樣品中抗原（蛋白質(zhì)、激素等）或抗體的技術(shù)。它依賴于抗體結(jié)合特定抗原的能力。用酶標(biāo)記抗體或抗原，所述酶的底物是轉(zhuǎn)化成可測(cè)量產(chǎn)物（顏色或熒光）的色原或熒光團(tuán)。|酶聯(lián)免疫吸附測(cè)定（ELISA）是使用固相（例如：微量滴定板）上包被有固定分子的抗原以進(jìn)行檢測(cè)。隨著時(shí)間的推移，科學(xué)家們已經(jīng)將術(shù)語ELISA擴(kuò)展到使用抗體包被固相的EIA。這就解釋了為什么我們使用包被抗體的EIA試劑盒也被稱為ELISA試劑盒。|雜質(zhì)評(píng)估是重組蛋白（包括治療性蛋白）的藥物開發(fā)生產(chǎn)過程中的關(guān)鍵步驟。來自介導(dǎo)蛋白質(zhì)表達(dá)的細(xì)胞的特定雜質(zhì)（稱為宿主細(xì)胞蛋白質(zhì)（HCP））被產(chǎn)生并且需要被去除。該試劑盒預(yù)期用于評(píng)估大腸桿菌的相對(duì)量。生產(chǎn)或研究生物制品中的大腸桿菌HCP。|該試劑盒中使用的多克隆抗體已經(jīng)針對(duì)幾種大腸桿菌菌株產(chǎn)生，并且針對(duì)它們識(shí)別大范圍的大腸桿菌而被特異性地選擇。coli蛋白。因此，該試劑盒可被認(rèn)為是通用的，并允許相對(duì)定量測(cè)定E。在許多類型的樣品中，例如從純化過程（HCP清除）、過程控制、質(zhì)量控制或產(chǎn)品放行中發(fā)出的樣品，可以檢測(cè)到大腸桿菌HCP。|使用該試劑盒，以ng/ml測(cè)量HCP濃度（HCP當(dāng)量從標(biāo)準(zhǔn)曲線外推）。通常，產(chǎn)品中的HCP含量最終以ng/mg表示，其中ng表示HCP質(zhì)量，mg表示產(chǎn)品質(zhì)量。請(qǐng)注意，與產(chǎn)品的濃度測(cè)量相反，HCP信號(hào)僅反映抗體結(jié)合，并不嚴(yán)格反映HCP的質(zhì)量。|已使用復(fù)溶HCP樣品成功驗(yàn)證了該試劑盒的回收率和精密度，并針對(duì)不同的最終產(chǎn)品進(jìn)行了檢測(cè)。鑒于最終產(chǎn)品的多樣性，無法了解所有潛在的基質(zhì)效應(yīng)，建議您在實(shí)驗(yàn)室檢測(cè)試劑盒與您自己的HCP樣本一起使用的適用性。|該試劑盒應(yīng)作為完整HCP分析的一部分使用。
- 產(chǎn)品特點(diǎn)
- 該宿主細(xì)胞蛋白（大腸桿菌）ELISA試劑盒,Host Cell Proteins (E.coli) ELISA kit產(chǎn)品產(chǎn)品特點(diǎn)詳情查看產(chǎn)品說明書。
- 保存建議
- Store at +4degrees; shelf life 6 months maximum after production
- 其他
- 法國(guó)Bertin Technologies公司是一家大型儀器設(shè)備及試劑供應(yīng)商，擁有50多年的歷史，產(chǎn)品涉及衛(wèi)生、環(huán)境、安全甚至軍工等諸多領(lǐng)域。Bertin Bioreagent是Bertin Technologies集團(tuán)的生物試劑部門，主要是致力于研發(fā)與炎癥，氧化損傷，內(nèi)分泌學(xué)，糖尿病，肥胖癥，高血壓，精神藥理學(xué)，癌癥，哮喘，朊病毒疾病，藥代動(dòng)力學(xué)和代謝等相關(guān)的化學(xué)試劑 ，蛋白，底物，抗體及檢測(cè)試劑盒等產(chǎn)品。 Bertin Instruments是Bertin Technologies集團(tuán)的儀器部門，其中旗艦產(chǎn)品Precellys系列多功能生物樣品勻漿器是全世界各個(gè)實(shí)驗(yàn)室的明星產(chǎn)品，截至目前累計(jì)有15000+的引文！
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- 注意
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該頁面的中文產(chǎn)品信息的翻譯，僅供參考。準(zhǔn)確的產(chǎn)品信息請(qǐng)以廠家的英文說明書為準(zhǔn)。下單前，請(qǐng)瀏覽說明書確認(rèn)。
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