該微管結(jié)合蛋白 Spin Down 檢測試劑盒產(chǎn)品是Cytoskeleton品牌原裝進(jìn)口,Cytoskeleton品牌是細(xì)胞骨架研究專家,專注于生物化學(xué)和細(xì)胞過程研究中的純化蛋白和便捷試劑盒開發(fā)與生產(chǎn)。艾美捷作為中國區(qū)域的總代理,是行業(yè)中少有的致力于服務(wù)客戶,幫助客戶,且擁有獨(dú)立專業(yè)技術(shù)支持團(tuán)隊(duì)的高科技生物企業(yè)。可以為您提供及時(shí)的咨詢響應(yīng),專業(yè)的產(chǎn)品和解決方案支持,穩(wěn)健快捷的交貨周期,優(yōu)質(zhì)放心的售后服務(wù)。我們致力于為您提供有價(jià)值的產(chǎn)品和服務(wù),在意您的成功!
產(chǎn)品描述
收到該微管結(jié)合蛋白 Spin Down 檢測試劑盒Microtubule Binding Protein Spin Down Assay Kit產(chǎn)品后,查看廠家提供的產(chǎn)品說明書詳細(xì)介紹。
產(chǎn)品特點(diǎn)
This assay allows the identification of proteins that will bind to microtubules (MTs) in vitro. The assay relies on the fact that MTs will pellet when centrifuged at 100,000 x g. Therefore, any protein that is associated with the MTs will pellet with them during centrifugation. A simple SDS-PAGE analysis of the supernatant versus pellet fraction will identify if a protein is able to associate with MTs. The assay description given in this manual is for recombinantly expressed “test” proteins, however, the assay can be adapted for cell lysates or in vitro translation products.
It should be noted that in vivo confirmation of MT association should be obtained in order to confirm that the protein can be classified as a MAP. This association need not occur throughout the whole cell cycle and may even be developmentally regulated, indeed transient association of MAPs with microtubules is the norm rather than the exception.