Arf1 Pulldown 活化檢測(cè)試劑盒-Arf1 Pulldown Activation Assay Kit代理/參數(shù)/廠家-細(xì)胞分析試劑盒-艾美捷科技有限公司

PRODUCT CENTER

細(xì)胞分析試劑盒

產(chǎn)品中心

當(dāng)前位置：首頁 > 產(chǎn)品中心 > 細(xì)胞生物學(xué)產(chǎn)品 > 細(xì)胞分析試劑盒

中文名稱: Arf1 Pulldown 活化檢測(cè)試劑盒

英文名字: Arf1 Pulldown Activation Assay Kit

供應(yīng)商: Cytoskeleton

產(chǎn)品貨號(hào): BK032-S

產(chǎn)品報(bào)價(jià): ￥詢價(jià)/KIT20assays

產(chǎn)品說明書: 點(diǎn)擊查看

購買方式: Cytoskeleton中國區(qū)現(xiàn)貨中心已經(jīng)建立，大部分產(chǎn)品中國有現(xiàn)貨庫存。銀行轉(zhuǎn)賬、電匯、支票、現(xiàn)金，在線支付寶及網(wǎng)銀支付，或直接與我們電話聯(lián)系400-6800-868

產(chǎn)品新聞

背景資料: 該Arf1 Pulldown 活化檢測(cè)試劑盒產(chǎn)品是Cytoskeleton品牌原裝進(jìn)口，Cytoskeleton品牌是細(xì)胞骨架研究專家，專注于生物化學(xué)和細(xì)胞過程研究中的純化蛋白和便捷試劑盒開發(fā)與生產(chǎn)。艾美捷作為中國區(qū)域的總代理，是行業(yè)中少有的致力于服務(wù)客戶，幫助客戶，且擁有獨(dú)立專業(yè)技術(shù)支持團(tuán)隊(duì)的高科技生物企業(yè)。可以為您提供及時(shí)的咨詢響應(yīng)，專業(yè)的產(chǎn)品和解決方案支持，穩(wěn)健快捷的交貨周期，優(yōu)質(zhì)放心的售后服務(wù)。我們致力于為您提供有價(jià)值的產(chǎn)品和服務(wù)，在意您的成功！

產(chǎn)品描述: 收到該Arf1 Pulldown 活化檢測(cè)試劑盒Arf1 Pulldown Activation Assay Kit產(chǎn)品后，查看廠家提供的產(chǎn)品說明書詳細(xì)介紹。

產(chǎn)品特點(diǎn): The mammalian ADP-ribosylation factor (Arf) subfamily of Ras-related small G-proteins was originally named for the ability to stimulate cholera toxin mediated ADP-ribosylation of the Gsα subunit utilized by many GPCRs (1). The Arf GTPases have been grouped into three classes based on their size and amino acid similarity (2): class I (Arf1 and Arf3), class II (Arf4 and Arf5) and class III (Arf6). Arf1 functions in the anterograde (and retrograde) transport of proteins from the endoplasmic reticulum (ER) through the Golgi apparatus to the plasma membrane by activating lipid modifying enzymes and recruiting proteins needed to promote secretory vesicle development, scission, and transport between secretory compartments (3, 4, and reviewed in 5). Arf1 has also been shown to function in the maintenance of structural integrity of the Golgi and ER (6). Arf1, like other small G-proteins, cycles between the inactive GDP-bound and the active GTP-bound states. The preferential association of effector proteins with the GTP-bound over the GDP-bound state of Arf1 provides the basis for Arf1’s function in the cell. This highly specific association of effector proteins with Arf1-GTP has been exploited to develop affinity precipitation assays to monitor Arf1 activation (7). Cytoskeleton’s Arf1 Activation Assay Biochem Kit? utilizes the Arf1 protein binding domain (PBD) of the effector protein GGA3 (Golgi-localized γ-ear containing, Arf-binding protein 3), which has been shown to specifically bind the GTP-bound form of Arf1 (7, 8). We have covalently conjugated purified GGA3-PBD (amino acids 1-316) expressed in E. coli to the colored sepharose beads provided in this kit (Figure 1). Using these beads, the researcher is able to “pull-down” Arf1-GTP and quantify the level of active Arf1 with a subsequent Western blotting step using the Arf1 specific antibody provided in this kit. This assay provides a simple means of analyzing cellular Arf1 activation levels in a variety of systems. A typical Arf1 pull-down assay is shown in Figure 2 below using either GTPgS and GDP loaded MDCK cell extracts or extracts from C2C12 myoblasts that have been induced to differentiate by serum starvation.

保存建議: 廠家推薦常溫運(yùn)輸，收到產(chǎn)品后建議保存于4°C。

其他: Cytoskeleton公司成立于1993年，專注于生物化學(xué)和細(xì)胞過程研究中的純化蛋白和便捷試劑盒開發(fā)與生產(chǎn)。公司提供藥物篩選、信號(hào)轉(zhuǎn)導(dǎo)、蛋白質(zhì)轉(zhuǎn)錄后修飾（PTM）、細(xì)胞骨架研究相關(guān)的系列試劑盒和產(chǎn)品，尤其以細(xì)胞骨架相關(guān)研究見長，既能滿足于樣品較少的科學(xué)研究，也可以用于小規(guī)模篩選研究和高通量大規(guī)模篩選研究。此外，公司還提供微管蛋白，肌動(dòng)蛋白，小G蛋白，GAPs，GEFs等現(xiàn)有產(chǎn)品的藥物篩選服務(wù)。