Rac1 Pulldown 活化檢測試劑盒現貨-Rac1 Pulldown Activation Assay Kit代理/參數/廠家-細胞分析試劑盒-艾美捷科技有限公司

PRODUCT CENTER

細胞分析試劑盒

產品中心

當前位置：首頁 > 產品中心 > 細胞生物學產品 > 細胞分析試劑盒

中文名稱: Rac1 Pulldown 活化檢測試劑盒現貨

英文名字: Rac1 Pulldown Activation Assay Kit

供應商: Cytoskeleton

產品貨號: BK035

產品報價: ￥詢價/KIT50assays

產品說明書: 點擊查看

購買方式: Cytoskeleton中國區(qū)現貨中心已經建立，大部分產品中國有現貨庫存。銀行轉賬、電匯、支票、現金，在線支付寶及網銀支付，或直接與我們電話聯系400-6800-868

產品新聞

背景資料: 該Rac1 Pulldown 活化檢測試劑盒產品是Cytoskeleton品牌原裝進口，Cytoskeleton品牌是細胞骨架研究專家，專注于生物化學和細胞過程研究中的純化蛋白和便捷試劑盒開發(fā)與生產。艾美捷作為中國區(qū)域的總代理，是行業(yè)中少有的致力于服務客戶，幫助客戶，且擁有獨立專業(yè)技術支持團隊的高科技生物企業(yè)。可以為您提供及時的咨詢響應，專業(yè)的產品和解決方案支持，穩(wěn)健快捷的交貨周期，優(yōu)質放心的售后服務。我們致力于為您提供有價值的產品和服務，在意您的成功！

產品描述: 收到該Rac1 Pulldown 活化檢測試劑盒Rac1 Pulldown Activation Assay Kit產品后，查看廠家提供的產品說明書詳細介紹。

產品特點: The Rho switch operates by alternating between an active, GTP-bound state and an inactive, GDP-bound state. Understanding the mechanisms that regulate activation / inactivation of the GTPases is of obvious biological significance and is a subject of intense investigation. The fact that many Rho family effector proteins will specifically recognize the GTP bound form of the protein has been exploited experimentally to develop a powerful affinity purification assay that monitors Rac and Cdc42 protein activation. The assay uses the Cdc42/Rac Interactive Binding (CRIB) region (also called the p21 Binding Domain, PBD) of the Cdc42 / Rac effector protein, p21 activated kinase 1 (PAK). The CRIB/PBD protein motif has been shown to bind specifically to the GTP-bound form of Rac and/or Cdc42 proteins. The fact that the PBD region of PAK has a high affinity for both GTP-Rac and GTP-Cdc42 and that PAK binding results in a significantly reduced intrinsic and catalytic rate of hydrolysis of both Rac and Cdc42 make it an ideal tool for affinity purification of GTP-Rac and GTP-Cdc42 from cell lysates. The PAK-PBD protein supplied in this kit corresponds to residues 67-150. This includes the highly conserved CRIB region (aa 74-88) plus sequences required for the high affinity interaction with GTP-Rac and GTP-Cdc42. The PAK-PBD is in the form of a GST fusion protein, which allows one to "pull-down" the PAK-PBD/GTP-Rac (or GTP-Cdc42) complex with glutathione affinity beads. The assay therefore provides a simple means of quantitating Rac or Cdc42 activation in cells. The amount of activated Rac is determined by a Western blot using a Rac-specific antibody.

保存建議: 廠家推薦常溫運輸，收到產品后建議保存于4°C。

其他: Cytoskeleton公司成立于1993年，專注于生物化學和細胞過程研究中的純化蛋白和便捷試劑盒開發(fā)與生產。公司提供藥物篩選、信號轉導、蛋白質轉錄后修飾（PTM）、細胞骨架研究相關的系列試劑盒和產品，尤其以細胞骨架相關研究見長，既能滿足于樣品較少的科學研究，也可以用于小規(guī)模篩選研究和高通量大規(guī)模篩選研究。此外，公司還提供微管蛋白，肌動蛋白，小G蛋白，GAPs，GEFs等現有產品的藥物篩選服務。