ELIPA ATP酶活性檢測(cè)試劑盒（酶聯(lián)無(wú)機(jī)磷酸鹽測(cè)定）-ATPase ELIPA (Enzyme Linked Inorganic Phosphate Assay)代理/參數(shù)/廠家-細(xì)胞分析試劑盒-艾美捷科技有限公司

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中文名稱: ELIPA ATP酶活性檢測(cè)試劑盒（酶聯(lián)無(wú)機(jī)磷酸鹽測(cè)定）

英文名字: ATPase ELIPA (Enzyme Linked Inorganic Phosphate Assay)

供應(yīng)商: Cytoskeleton

產(chǎn)品貨號(hào): BK051

產(chǎn)品報(bào)價(jià): ￥已停產(chǎn)

產(chǎn)品說(shuō)明書: 點(diǎn)擊查看

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背景資料: 該ELIPA ATP酶活性檢測(cè)試劑盒（酶聯(lián)無(wú)機(jī)磷酸鹽測(cè)定）產(chǎn)品是Cytoskeleton品牌原裝進(jìn)口，Cytoskeleton品牌是細(xì)胞骨架研究專家，專注于生物化學(xué)和細(xì)胞過(guò)程研究中的純化蛋白和便捷試劑盒開(kāi)發(fā)與生產(chǎn)。艾美捷作為中國(guó)區(qū)域的總代理，是行業(yè)中少有的致力于服務(wù)客戶，幫助客戶，且擁有獨(dú)立專業(yè)技術(shù)支持團(tuán)隊(duì)的高科技生物企業(yè)。可以為您提供及時(shí)的咨詢響應(yīng)，專業(yè)的產(chǎn)品和解決方案支持，穩(wěn)健快捷的交貨周期，優(yōu)質(zhì)放心的售后服務(wù)。我們致力于為您提供有價(jià)值的產(chǎn)品和服務(wù)，在意您的成功！

產(chǎn)品描述: 收到該ELIPA ATP酶活性檢測(cè)試劑盒（酶聯(lián)無(wú)機(jī)磷酸鹽測(cè)定）ATPase ELIPA (Enzyme Linked Inorganic Phosphate Assay)產(chǎn)品后，查看廠家提供的產(chǎn)品說(shuō)明書詳細(xì)介紹。

產(chǎn)品特點(diǎn): There are a multitude of enzymes that hydrolyze ATP or GTP to form ADP or GDP and inorganic phosphate (Pi). The Enzyme Linked Inorganic Phosphate Assay (ELIPA) from Cytoskeleton Inc. allows one to measure the phosphate released during hydrolysis on a real time basis. Thus a kinetic assay technique is produced for your enzyme’s activity. Particular purified enzymes that are applicable to this analysis are signaling phosphatases, apyrase, kinesin motors (see Fig. 1), metabolic enzymes, membrane transporters and alkaline phosphatase. Generally this assay is useful for enzymes with Kcat above 0.1, if your enzyme or the family of enzymes has a lower Kcat then the CytoPhosTM Phosphate Assay (Cat. # BK054) is useful. If your enzyme has a very low activity i.e. small G-proteins, the non-radioactive GAP assay (Cat. # BK105) or the radioactive alternative, EasyRad Phosphate Assay (Cat. # BK055) is ideal. The assay is an adaptation of a method originally described by Webb for the measurement of glycerol kinase plus D-glyceraldehyde ATPase activity and for actin activated myosin ATPase (1). The assay is based upon an absorbance shift (330-360 nm) that occurs when 2-amino-6-mercapto-7-methylpurine ribonucleoside (MESG) is catalytically converted to 2-amino-6-mercapto-7-methyl purine in the presence of inorganic phosphate (Pi). The reaction is catalyzed by purine nucleoside phosphorylase (PNP). One molecule of inorganic phosphate will yield one molecule of 2-amino-6-mercapto-7-methyl purine in an essentially irreversible reaction (2). Thus, the absorbance at 360 nm is directly proportional to the amount of Pi generated in the reaction.

保存建議: 廠家推薦常溫運(yùn)輸，收到產(chǎn)品后建議保存于4°C。

其他: Cytoskeleton公司成立于1993年，專注于生物化學(xué)和細(xì)胞過(guò)程研究中的純化蛋白和便捷試劑盒開(kāi)發(fā)與生產(chǎn)。公司提供藥物篩選、信號(hào)轉(zhuǎn)導(dǎo)、蛋白質(zhì)轉(zhuǎn)錄后修飾（PTM）、細(xì)胞骨架研究相關(guān)的系列試劑盒和產(chǎn)品，尤其以細(xì)胞骨架相關(guān)研究見(jiàn)長(zhǎng)，既能滿足于樣品較少的科學(xué)研究，也可以用于小規(guī)模篩選研究和高通量大規(guī)模篩選研究。此外，公司還提供微管蛋白，肌動(dòng)蛋白，小G蛋白，GAPs，GEFs等現(xiàn)有產(chǎn)品的藥物篩選服務(wù)。