GEF 轉(zhuǎn)化活性檢測試劑盒-GEF Exchange Assay代理/參數(shù)/廠家-細胞分析試劑盒-艾美捷科技有限公司

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中文名稱: GEF 轉(zhuǎn)化活性檢測試劑盒

英文名字: GEF Exchange Assay

供應商: Cytoskeleton

產(chǎn)品貨號: BK100

產(chǎn)品報價: ￥詢價/KIT60-300assays

產(chǎn)品說明書: 點擊查看

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背景資料: 該GEF 轉(zhuǎn)化活性檢測試劑盒產(chǎn)品是Cytoskeleton品牌原裝進口，Cytoskeleton品牌是細胞骨架研究專家，專注于生物化學和細胞過程研究中的純化蛋白和便捷試劑盒開發(fā)與生產(chǎn)。艾美捷作為中國區(qū)域的總代理，是行業(yè)中少有的致力于服務客戶，幫助客戶，且擁有獨立專業(yè)技術(shù)支持團隊的高科技生物企業(yè)。可以為您提供及時的咨詢響應，專業(yè)的產(chǎn)品和解決方案支持，穩(wěn)健快捷的交貨周期，優(yōu)質(zhì)放心的售后服務。我們致力于為您提供有價值的產(chǎn)品和服務，在意您的成功！

產(chǎn)品描述: 收到該GEF 轉(zhuǎn)化活性檢測試劑盒GEF Exchange Assay產(chǎn)品后，查看廠家提供的產(chǎn)品說明書詳細介紹。

產(chǎn)品特點: The Ras superfamily of small GTPases (such as Ras, Rho, Rab, Arf and Ran proteins) serve as binary switches cycling between a GDP-bound “OFF state” and a GTP-bound “ON state”. In cells, the cycling between the two states is mainly controlled by two types of regulatory proteins: the activating Guanine nucleotide Exchange Factors (GEFs) and the inactivating GTPase Activating Proteins (GAPs). GEFs function by catalyzing the exchange of nucleotide on their target GTPases. The GEF will bind to a nucleotide-bound GTPase, which causes the bound nucleotide to be released, thus resulting in a nucleotide free GEF-GTPase reaction intermediate. This nucleotide free complex will then take up a new nucleotide after which the GEF is released from the GTPase. Because GEFs typically have a higher affinity for GDP-bound GTPases than for the corresponding GTP-bound GTPase and the intracellular ratio of GTP to GDP is about 10:1, GEFs will drive the exchange from GDP-bound GTPases to GTP-bound GTPases The fluorophore based assay in this kit is suitable for measuring nucleotide exchange on GTPases in either 96-well or 384-well format. The kit measures the uptake of the fluorescent nucleotide analog N-methylanthraniloyl-GTP (mant-GTP) into GTPases. The uptake can be measured due to the spectroscopic difference between free and GTPase-bound mant-GTP. As mant-GTP gets bound in the nucleotide binding pocket of a GTPase, its fluorescence (ex: 360 nm, em: 440 nm) increases dramatically (1, 2). Therefore, the enhancement of mant-GTP fluorescent intensity in the presence of a small GTPase indicates nucleotide uptake (or exchange for already bound nucleotide) by the GTPase. This kit contains all the buffers and reagents needed for the assay, a 96 and a 384-well plate, as well as human Cdc42, Rac1 and RhoA GTPases and the GEF domain of Dbs as a positive control GEF. Dbs is an effective GEF for Cdc42 and RhoA but shows only very low GEF activity for Rac1 (see Figs 1 & 2). The kit provides enough material for 20 assays for each GTPase in 96-well format or 100 assays for each GTPase in 384-well format.

保存建議: 廠家推薦常溫運輸，收到產(chǎn)品后建議保存于4°C。

其他: Cytoskeleton公司成立于1993年，專注于生物化學和細胞過程研究中的純化蛋白和便捷試劑盒開發(fā)與生產(chǎn)。公司提供藥物篩選、信號轉(zhuǎn)導、蛋白質(zhì)轉(zhuǎn)錄后修飾（PTM）、細胞骨架研究相關的系列試劑盒和產(chǎn)品，尤其以細胞骨架相關研究見長，既能滿足于樣品較少的科學研究，也可以用于小規(guī)模篩選研究和高通量大規(guī)模篩選研究。此外，公司還提供微管蛋白，肌動蛋白，小G蛋白，GAPs，GEFs等現(xiàn)有產(chǎn)品的藥物篩選服務。