3-Probe bone affinity sampler(56-ROX-RIS (24 nmol) + 56-FAM-dRIS (24 nmol) + AF647-RISPC (24 nmol)),This sampler kit includes three imaging probes with different spectroscopic properties and high, medium and low bone affinities (in the order of 5(6)-ROX-RIS > 5(6)-FAM-dRIS > AF647-RISPC). All three probes include bisphosphonate or phosphonocarboxylate molecular skeleton, thus have higher affinity than calcein (the fluorescent molecule with the least bone affinity). The kit can be used for simultaneous detection with co-dosing and studies on effects of bone affinity.n nSpectroscopic properties of 5(6)-ROX-RIS:nMaximum absorption wavelength (λmax (abs)): 580 nmnMaximum emission wavelength (λmax (em)): 605 nmnExtinction coefficient (M-1cm-1): 73,000 (0.1 M phosphate buffer, pH 8.0)n nSpectroscopic properties of 5(6)-FAM-dRIS:nMaximum absorption wavelength (λmax (abs)): 493 nmnMaximum emission wavelength (λmax (em)): 518 nmnExtinction coefficient (M-1cm-1): 73,000 (0.1 M phosphate buffer, pH 7.2)nSpectroscopic properties of AF647-RISPC:nMaximum absorption wavelength (λmax (abs)): 648 nmnMaximum emission wavelength (λmax (em)): 666 nmnExtinction coefficient (M-1cm-1): 240,000 (0.1 M phosphate buffer, pH 7.0)