MB 660R DBCO is a bright and very photostable probe used for imaging of azide-containing biomolecules without the need for copper catalyst. MB 660R DBCO reacts with azides via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. In application where the presence of copper is a concern MB 66oR DBCO is an ideal alternative to copper requiring fluorescent alkynes.nnMB 660R is a bright and photostable far-red dye that emits fluorescence at about 685 nm in the borderline spectral region between far-red and near-IR. Although the absorption maximum is at around 665 nm, this dye can be sufficiently excited by the 633 or 635 nm laser. MB 660R dye is water soluble and pH-insensitive from pH 4 to pH 10. MB 660R is a rhodamine-based dye, and like rhodamine dyes in general, it is very bright and exceptionally photostable . The superior photostability and excellent brightness of MB 660R makes this dye an ideal choice for confocal microscopy single molecule imaging and other applications that demand both brightness and photostability.nnnMB 660R dye spectrally is almost identical to Alexa Fluor? 660 and CF? 660R Dye and can be used a less expensive alternative to these dyes.nnMB 660R DBCO reagent is not suitable for staining intracellular components of fixed and permeabilized cells due to high backgrounds.nnAlexa Fluor? is a registered trademark of Thermo Fisher Scientific. CF? Dye is a registered trademark of Biotium.
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Click Chemistry Tools(CCT)公司位于美國亞利桑那州斯科茨代爾(Scottsdale,AZ)。自公司成立以來,一致致力于不斷開發(fā)和擴展一系列連接產(chǎn)品。他們利用特別新的研究成果研發(fā)生產(chǎn)出新穎的交聯(lián)和標記試劑。產(chǎn)品被廣泛用于藥物研發(fā)、癌癥治療以及各類診斷技術(shù)等。此外,CCT還可以提供個性化的定制產(chǎn)品,已滿足不同用戶的需求。目前CCT的產(chǎn)品線主要包括:Azides(Biotin-Azides)試劑、Bis-sulfone交聯(lián)試劑、DBCO試劑、TCO試劑、Terminal Alkyne試劑、Tetrazine試劑、標記試劑盒以及分選用底物等。nFluoroprobes公司位于美國亞利桑那州斯科茨代爾(Scottsdale, AZ),自成立以來,一直致力于不斷開發(fā)一系列的連接(linker)產(chǎn)品。利用特別新的研究成果研發(fā)生產(chǎn)出各類新穎的交聯(lián)和標記試劑。其產(chǎn)品被廣泛用于藥物研發(fā)與篩選、疾病治療方法和診斷技術(shù)等領(lǐng)域。此外,F(xiàn)luoroprobes還可以根據(jù)客戶的個性化需求提供各類定制連接產(chǎn)品。代表產(chǎn)品有Classic Fluorescent Dyes, AZDye, Cyanine Dyes, IR Dyes, MB dyes以及一些標記試劑盒等。nQuanta Biodesign公司位于美國中部的俄亥俄州,成立于1997年。也是專注于生產(chǎn)研發(fā)各類標記產(chǎn)品的品牌之一。其研發(fā)的產(chǎn)品與dPEG技術(shù)相結(jié)合,生產(chǎn)出他們獨有的專利產(chǎn)品dPEG Building Blocks and Linkers,被廣泛用于細胞治療和分子診斷等研究領(lǐng)域。另外,還可為廣大科研工作者提供多種化學修飾試劑以及傳統(tǒng)Liner等。n目前上述三個品牌都已被被Vectorlabs收購并整合到Vectorlabs產(chǎn)品中