AZDye? 555 NHS Ester is a water-soluble, bright orange-fluorescent dye with excitation ideally suited for the 532 nm or 555 nm laser lines and visualized with TRITC (tetramethylrhodamine) filter sets. AZDye? 555 conjugates of antibodies, peptides, and proteins are pH insensitive from pH 4 to pH 10. Proteins can be labeled with AZDye 555 NHS Ester at high molar ratios without significant self-quenching, leading to brighter conjugates and more sensitive detection. AZDye? 555 conjugates ideally suited for detection of low abundance targets.nnThe NHS Ester reacts specifically and efficiently with a primary amine at pH 7-9 to form a stable, covalent amide bond. The NHS ester (or succinimidyl ester) is the most popular tool for conjugating dyes to the primary amines of protein or antibody (Lys), amine-modified oligonucleotides, and other amine-containing molecules.nnAZDye? 555 is spectrally is almost identical to Cy3 Dye, Alexa Fluor? 555, CF? 555 Dye, or any other Cyanine3 based fluorescent dyes.nnAlexa Fluor? 555 is a registered trademark of Thermo Fisher Scientific.
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Click Chemistry Tools(CCT)公司位于美國(guó)亞利桑那州斯科茨代爾(Scottsdale,AZ)。自公司成立以來(lái),一致致力于不斷開(kāi)發(fā)和擴(kuò)展一系列連接產(chǎn)品。他們利用特別新的研究成果研發(fā)生產(chǎn)出新穎的交聯(lián)和標(biāo)記試劑。產(chǎn)品被廣泛用于藥物研發(fā)、癌癥治療以及各類(lèi)診斷技術(shù)等。此外,CCT還可以提供個(gè)性化的定制產(chǎn)品,已滿(mǎn)足不同用戶(hù)的需求。目前CCT的產(chǎn)品線(xiàn)主要包括:Azides(Biotin-Azides)試劑、Bis-sulfone交聯(lián)試劑、DBCO試劑、TCO試劑、Terminal Alkyne試劑、Tetrazine試劑、標(biāo)記試劑盒以及分選用底物等。nFluoroprobes公司位于美國(guó)亞利桑那州斯科茨代爾(Scottsdale, AZ),自成立以來(lái),一直致力于不斷開(kāi)發(fā)一系列的連接(linker)產(chǎn)品。利用特別新的研究成果研發(fā)生產(chǎn)出各類(lèi)新穎的交聯(lián)和標(biāo)記試劑。其產(chǎn)品被廣泛用于藥物研發(fā)與篩選、疾病治療方法和診斷技術(shù)等領(lǐng)域。此外,F(xiàn)luoroprobes還可以根據(jù)客戶(hù)的個(gè)性化需求提供各類(lèi)定制連接產(chǎn)品。代表產(chǎn)品有Classic Fluorescent Dyes, AZDye, Cyanine Dyes, IR Dyes, MB dyes以及一些標(biāo)記試劑盒等。nQuanta Biodesign公司位于美國(guó)中部的俄亥俄州,成立于1997年。也是專(zhuān)注于生產(chǎn)研發(fā)各類(lèi)標(biāo)記產(chǎn)品的品牌之一。其研發(fā)的產(chǎn)品與dPEG技術(shù)相結(jié)合,生產(chǎn)出他們獨(dú)有的專(zhuān)利產(chǎn)品dPEG Building Blocks and Linkers,被廣泛用于細(xì)胞治療和分子診斷等研究領(lǐng)域。另外,還可為廣大科研工作者提供多種化學(xué)修飾試劑以及傳統(tǒng)Liner等。n目前上述三個(gè)品牌都已被被Vectorlabs收購(gòu)并整合到Vectorlabs產(chǎn)品中