中文名稱

NHS-dPEG?生物素酶抗性生物素-100 mg

英文名字

NHS-dPEG??-biotinidase resistant biotin-100 mg

供應(yīng)商

Click Chemistry Tools

產(chǎn)品貨號

CCT-QBD-10202-100

產(chǎn)品報價

￥詢價/100mg

產(chǎn)品說明書

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購買方式

銀行轉(zhuǎn)賬、電匯、支票、現(xiàn)金，在線支付寶及網(wǎng)銀支付，或直接與我們電話聯(lián)系。本公司所有產(chǎn)品僅供科研使用，不用于臨床診斷。

產(chǎn)品新聞

背景資料

NHS-dPEG?4-生物素酶抗性生物素，產(chǎn)品編號QBD-10202，是一種經(jīng)修飾以抵抗生物素酶水解的水溶性生物素化試劑(EC 3.5.1.12)。該產(chǎn)品將生物素酰-2-氨基丁酸與單分子量離散聚乙二醇(dPEG?)連接體的N-羥基琥珀酰亞胺(NHS)酯結(jié)合。NHS-dPEG?4-生物素酶抗性生物素保留了生物素對抗生物素蛋白和鏈霉抗生物素蛋白的典型高親和力。然而，經(jīng)修飾的生物素阻止生物素酶的再循環(huán)。該產(chǎn)品可用于組織樣品的ELISA和抗體預(yù)靶向等應(yīng)用，其中內(nèi)源性生物素酶可回收生物素。

產(chǎn)品描述

NHS-dPEG?4-biotinidase-resistant biotin, product number QBD-10202, is a water-soluble biotinylation reagent modified to resist hydrolysis by biotinidase (EC 3.5.1.12). This product combines Biotinoyl-2-aminobutyric acid with the N-hydroxysuccinimidyl (NHS) ester of a single molecular weight, discrete polyethylene glycol (dPEG?) linker. NHS-dPEG?4-biotinidase-resistant biotin retains biotin’s typical high affinity for avidin and streptavidin. However, the modified biotin prevents recycling by biotinidase. This product is useful in applications such as ELISAs of tissue samples and antibody pretargeting, where endogenous biotinidase can recycle biotin.nBiotinidase is an amidohydrolase that breaks the covalent bond between biotin and amino acids such as lysine (i.e., biocytin). In vivo, biotinidase recycles biotin. Although necessary for healthy bodily function, biotinidase potentially creates problems when biotinylated proteins are used in serological assays, since endogenous biotinidase removes the biotin from these proteins.nDr. D. Scott Wilbur and colleagues at the University of Washington in Seattle developed biotinidase-resistant biotin in 2006 to facilitate in vivo antibody pretargeting. However, to improve the product’s water solubility, a short amino-dPEG?4-propionic acid linker was conjugated to the biotinidase-resistant biotin and subsequently functionalized as an NHS ester.nLike our other dPEG? biotinylation products, NHS-dPEG?4-biotinidase-resistant biotin will not cause aggregation and precipitation of biomolecules to which it is conjugated, and the dPEG? spacer reduces non-specific binding.nNHS-dPEG?4-biotinidase-resistant biotin resists amide hydrolysis because of the non-natural amino acid to which it is conjugated. It is potentially beneficial for applications using serological fluids that contain endogenous biotinidase. Useful roles for NHS-dPEG?4-biotinidase-resistant biotin include the following:ntherapeutic applications such as antibody pretargeting with biotin-streptavidin systems;nsupramolecular constructs (e.g., nanoparticles) used in vivo that depend on biotin-(strept)avidin affinity to maintain structural integrity; and,nELISAs of serological fluids and tissue samples using biotin-(strept)avidin affinity.

產(chǎn)品形式

白色至類白色固體

保存建議

其他

Click Chemistry Tools（CCT）公司位于美國亞利桑那州斯科茨代爾（Scottsdale,AZ）。自公司成立以來，一致致力于不斷開發(fā)和擴展一系列連接產(chǎn)品。他們利用特別新的研究成果研發(fā)生產(chǎn)出新穎的交聯(lián)和標記試劑。產(chǎn)品被廣泛用于藥物研發(fā)、癌癥治療以及各類診斷技術(shù)等。此外，CCT還可以提供個性化的定制產(chǎn)品，已滿足不同用戶的需求。目前CCT的產(chǎn)品線主要包括：Azides（Biotin-Azides）試劑、Bis-sulfone交聯(lián)試劑、DBCO試劑、TCO試劑、Terminal Alkyne試劑、Tetrazine試劑、標記試劑盒以及分選用底物等。nFluoroprobes公司位于美國亞利桑那州斯科茨代爾(Scottsdale, AZ)，自成立以來，一直致力于不斷開發(fā)一系列的連接(linker)產(chǎn)品。利用特別新的研究成果研發(fā)生產(chǎn)出各類新穎的交聯(lián)和標記試劑。其產(chǎn)品被廣泛用于藥物研發(fā)與篩選、疾病治療方法和診斷技術(shù)等領(lǐng)域。此外，F(xiàn)luoroprobes還可以根據(jù)客戶的個性化需求提供各類定制連接產(chǎn)品。代表產(chǎn)品有Classic Fluorescent Dyes, AZDye, Cyanine Dyes, IR Dyes, MB dyes以及一些標記試劑盒等。nQuanta Biodesign公司位于美國中部的俄亥俄州，成立于1997年。也是專注于生產(chǎn)研發(fā)各類標記產(chǎn)品的品牌之一。其研發(fā)的產(chǎn)品與dPEG技術(shù)相結(jié)合，生產(chǎn)出他們獨有的專利產(chǎn)品dPEG Building Blocks and Linkers，被廣泛用于細胞治療和分子診斷等研究領(lǐng)域。另外，還可為廣大科研工作者提供多種化學修飾試劑以及傳統(tǒng)Liner等。n目前上述三個品牌都已被被Vectorlabs收購并整合到Vectorlabs產(chǎn)品中

注意

該頁面的中文產(chǎn)品信息的翻譯，僅供參考。準確的產(chǎn)品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。

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