中文名稱

MAL-dPEG

英文名字

MAL-dPEG???-NHS ester-1000 mg

供應商

Click Chemistry Tools

產品貨號

CCT-QBD-10284-1000

產品報價

￥詢價/1000mg

產品說明書

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購買方式

銀行轉賬、電匯、支票、現(xiàn)金，在線支付寶及網銀支付，或直接與我們電話聯(lián)系。本公司所有產品僅供科研使用，不用于臨床診斷。

產品新聞

背景資料

MAL-dPEG?12-NHS酯(產品編號QBD-10284)是一種交聯(lián)劑，可通過親水橋將巰基連接到游離胺上。巰基通過邁克爾加成反應與馬來酰亞胺基團反應。胺通過羧酸基團的N-羥基琥珀酰亞胺基(NHS)酯的親核取代與交聯(lián)劑形成酰胺鍵。交聯(lián)化合物上的馬來酰亞胺和NHS官能團位于長的離散長度聚乙二醇鏈(dPEG?)的任一端。

產品描述

er by nucleophilic substitution of the N-hydroxysuccinimidyl (NHS) ester of a carboxylic acid group. The maleimide and NHS functional groups on the crosslinking compound sit at either end of a long, discrete-length polyethylene glycol chain (dPEG?).nReactions that join free amines with free thiols are among the most popular, most useful crosslinking reactions in bioconjugate chemistry. These reactions require heterobifunctional reagents that bridge the two groups. Traditional crosslinkers are hydrophobic molecules. Vector Laboratories' dPEG? crosslinking products are water-soluble, amphiphilic, single molecular weight PEG compounds with discrete chain lengths.nThe conjugation of conventional hydrophobic crosslinking reagents to biomolecules almost inevitably triggers problems such as aggregation and precipitation of the conjugates. These problems do not occur with our water-soluble, non-immunogenic dPEG? crosslinkers.nBecause NHS esters hydrolyze readily in water or aqueous buffer, the NHS ester end of the molecule must conjugate to a target molecule before conjugating the maleimide end of the molecule. At pH 7.0 – 7.5, NHS esters react optimally with free amines. However, NHS esters can react with free amines with pH as low as 6.5. As the pH increases, the hydrolysis rate of the NHS ester increases. Indeed, at pH 8 and 25°C, the half-life of an NHS ester in aqueous media is one hour, while at pH 8.6 and 4°C, the half-life falls to ten (10) minutes.[3] Thus, we strongly discourage storing MAL-dPEG?12-NHS ester, product number QBD-10284, in water or aqueous buffer. Instead, we recommend that customers make new solutions of the product as needed, use them immediately, and discard unused solutions after use.nThe reaction of the maleimide end of MAL-dPEG?12-NHS ester with a sulfhydryl proceeds optimally at pH 6.5 – 7.5. Use the lowest reasonable pH within this range. Above pH 7.5, free amines compete with free thiols at the maleimide reaction site, which can cause confusing results. Moreover, at higher pH values, the maleimide ring may open to form unreactive maleamic acid.nThe use of MAL-dPEG?12-NHS ester, product number 10284, has been published in numerous scientific paper and patents. The following list highlights some of the more important uses of this product:ndevelopment of various imaging applications;ndevelopment of antibody-drug conjugates and extracellular antibody-drug conjugates;nmodification of diabodies and bispecific antibody fragments;ndevelopment of different types of assays for peptides, proteins, and oligonucleotides;nattachment of antibodies to atomic force microscopy probes;ncreation of a scaffold for HIV inhibitors;ndevelopment of biosensors; and,nsurface coating of nanoparticles.nAlso, in a radioimaging application, MAL-dPEG?12-NHS ester demonstrably improved both the biodistribution and image quality of a DOTA-conjugated diabody.

產品形式

無色至淺琥珀色油狀物;在儲存條件下可能凝固為白色至類白色蠟狀固體

保存建議

其他

Click Chemistry Tools（CCT）公司位于美國亞利桑那州斯科茨代爾（Scottsdale,AZ）。自公司成立以來，一致致力于不斷開發(fā)和擴展一系列連接產品。他們利用特別新的研究成果研發(fā)生產出新穎的交聯(lián)和標記試劑。產品被廣泛用于藥物研發(fā)、癌癥治療以及各類診斷技術等。此外，CCT還可以提供個性化的定制產品，已滿足不同用戶的需求。目前CCT的產品線主要包括：Azides（Biotin-Azides）試劑、Bis-sulfone交聯(lián)試劑、DBCO試劑、TCO試劑、Terminal Alkyne試劑、Tetrazine試劑、標記試劑盒以及分選用底物等。nFluoroprobes公司位于美國亞利桑那州斯科茨代爾(Scottsdale, AZ)，自成立以來，一直致力于不斷開發(fā)一系列的連接(linker)產品。利用特別新的研究成果研發(fā)生產出各類新穎的交聯(lián)和標記試劑。其產品被廣泛用于藥物研發(fā)與篩選、疾病治療方法和診斷技術等領域。此外，F(xiàn)luoroprobes還可以根據客戶的個性化需求提供各類定制連接產品。代表產品有Classic Fluorescent Dyes, AZDye, Cyanine Dyes, IR Dyes, MB dyes以及一些標記試劑盒等。nQuanta Biodesign公司位于美國中部的俄亥俄州，成立于1997年。也是專注于生產研發(fā)各類標記產品的品牌之一。其研發(fā)的產品與dPEG技術相結合，生產出他們獨有的專利產品dPEG Building Blocks and Linkers，被廣泛用于細胞治療和分子診斷等研究領域。另外，還可為廣大科研工作者提供多種化學修飾試劑以及傳統(tǒng)Liner等。n目前上述三個品牌都已被被Vectorlabs收購并整合到Vectorlabs產品中

注意

該頁面的中文產品信息的翻譯，僅供參考。準確的產品信息請以廠家的英文說明書為準。下單前，請瀏覽說明書確認。

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