MAL-dPEG -MAL-dPEG???-NHS ester-1000 mg代理/參數(shù)/廠家-生化試劑-艾美捷科技有限公司

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中文名稱: MAL-dPEG

英文名字: MAL-dPEG???-NHS ester-1000 mg

供應(yīng)商: Click Chemistry Tools

產(chǎn)品貨號(hào): CCT-QBD-10314-1000

產(chǎn)品報(bào)價(jià): ￥詢價(jià)/1000mg

產(chǎn)品說(shuō)明書: 點(diǎn)擊查看

購(gòu)買方式: 銀行轉(zhuǎn)賬、電匯、支票、現(xiàn)金，在線支付寶及網(wǎng)銀支付，或直接與我們電話聯(lián)系。本公司所有產(chǎn)品僅供科研使用，不用于臨床診斷。

產(chǎn)品新聞

背景資料: MAL-dPEG?24-NHS酯(產(chǎn)品編號(hào)QBD-10314)是一種交聯(lián)劑，可通過(guò)親水橋?qū)€基連接到游離胺上。巰基通過(guò)邁克爾加成反應(yīng)與馬來(lái)酰亞胺基團(tuán)反應(yīng)。胺通過(guò)羧酸基團(tuán)的N-羥基琥珀酰亞胺基(NHS)酯的親核取代與交聯(lián)劑形成酰胺鍵。交聯(lián)化合物上的馬來(lái)酰亞胺和NHS官能團(tuán)位于長(zhǎng)的離散長(zhǎng)度聚乙二醇鏈(dPEG?)的任一端。

產(chǎn)品描述: MAL-dPEG?24-NHS ester, product number QBD-10314, is a crosslinking reagent that joins a sulfhydryl to a free amine through a hydrophilic bridge. The sulfhydryl groups react with a maleimide group via a Michael addition reaction. The amines form amide bonds with the crosslinker by nucleophilic substitution of the N-hydroxysuccinimidyl (NHS) ester of a carboxylic acid group. The maleimide and NHS functional groups on the crosslinking compound sit at either end of a long, discrete-length polyethylene glycol chain (dPEG?).nReactions that join free amines with free thiols are among the most popular, most useful crosslinking reactions in bioconjugate chemistry. These reactions require heterobifunctional reagents that bridge the two groups. Traditional crosslinkers are hydrophobic molecules. Vector Laboratories' dPEG? crosslinking products are water-soluble, amphiphilic, single molecular weight PEG compounds with discrete chain lengths.nThe conjugation of conventional hydrophobic crosslinking reagents to biomolecules almost inevitably triggers problems such as aggregation and precipitation of the conjugates. These problems do not occur with our water-soluble, non-immunogenic dPEG? crosslinkers.nBecause NHS esters hydrolyze readily in water or aqueous buffer, the NHS ester end of the molecule must conjugate to a target molecule before conjugating the maleimide end of the molecule. At pH 7.0 – 7.5, NHS esters react optimally with free amines. However, NHS esters can react with free amines with pH as low as 6.5. As the pH increases, the hydrolysis rate of the NHS ester increases. Indeed, at pH 8 and 25°C, the half-life of an NHS ester in aqueous media is one hour, while at pH 8.6 and 4°C, the half-life falls to ten (10) minutes.[3] Thus, we strongly discourage storing MAL-dPEG?24-NHS ester, product number QBD-10314, in water or aqueous buffer. Instead, we recommend that customers make new solutions of the product as needed, use them immediately, and discard unused solutions after use.nThe reaction of the maleimide end of MAL-dPEG?24-NHS ester with a sulfhydryl proceeds optimally at pH 6.5 – 7.5. Use the lowest reasonable pH within this range. Above pH 7.5, free amines compete with free thiols at the maleimide reaction site, which can cause confusing results. Moreover, at higher pH values, the maleimide ring may open to form unreactive maleamic acid.nThe use of MAL-dPEG?24-NHS ester, product number QBD-10314, has been published in numerous scientific paper and patents. The following list highlights some of the more important uses of this product:nextending the in vivo half-life of a therapeutic diabody;ntargeted delivery of a cancer drug via polylysine dendrimers;ntargeted delivery of drugs to the lungs;ndevelopment of a blood purification device using magnetic nanoparticles;ndevelopment of a targeted therapeutic system using superparamagnetic nanoparticles;nimproved transfection of tumor cells by PEGylated liposomes;ndevelopment of immunosensors and biosensors;ninvestigation of an aptamer using atomic force microscopy;nattaching antibodies to atomic force microscopy probes;ndevelopment of molecular pincers;nimprovement of a peptidic ligand used for capture and purification of IgM; and,nsurface coating of nanoparticles.

產(chǎn)品形式: 白色至類白色固體

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其他: Click Chemistry Tools（CCT）公司位于美國(guó)亞利桑那州斯科茨代爾（Scottsdale,AZ）。自公司成立以來(lái)，一致致力于不斷開發(fā)和擴(kuò)展一系列連接產(chǎn)品。他們利用特別新的研究成果研發(fā)生產(chǎn)出新穎的交聯(lián)和標(biāo)記試劑。產(chǎn)品被廣泛用于藥物研發(fā)、癌癥治療以及各類診斷技術(shù)等。此外，CCT還可以提供個(gè)性化的定制產(chǎn)品，已滿足不同用戶的需求。目前CCT的產(chǎn)品線主要包括：Azides（Biotin-Azides）試劑、Bis-sulfone交聯(lián)試劑、DBCO試劑、TCO試劑、Terminal Alkyne試劑、Tetrazine試劑、標(biāo)記試劑盒以及分選用底物等。nFluoroprobes公司位于美國(guó)亞利桑那州斯科茨代爾(Scottsdale, AZ)，自成立以來(lái)，一直致力于不斷開發(fā)一系列的連接(linker)產(chǎn)品。利用特別新的研究成果研發(fā)生產(chǎn)出各類新穎的交聯(lián)和標(biāo)記試劑。其產(chǎn)品被廣泛用于藥物研發(fā)與篩選、疾病治療方法和診斷技術(shù)等領(lǐng)域。此外，F(xiàn)luoroprobes還可以根據(jù)客戶的個(gè)性化需求提供各類定制連接產(chǎn)品。代表產(chǎn)品有Classic Fluorescent Dyes, AZDye, Cyanine Dyes, IR Dyes, MB dyes以及一些標(biāo)記試劑盒等。nQuanta Biodesign公司位于美國(guó)中部的俄亥俄州，成立于1997年。也是專注于生產(chǎn)研發(fā)各類標(biāo)記產(chǎn)品的品牌之一。其研發(fā)的產(chǎn)品與dPEG技術(shù)相結(jié)合，生產(chǎn)出他們獨(dú)有的專利產(chǎn)品dPEG Building Blocks and Linkers，被廣泛用于細(xì)胞治療和分子診斷等研究領(lǐng)域。另外，還可為廣大科研工作者提供多種化學(xué)修飾試劑以及傳統(tǒng)Liner等。n目前上述三個(gè)品牌都已被被Vectorlabs收購(gòu)并整合到Vectorlabs產(chǎn)品中