中文名稱

MAL-dPEG TFP ester-1000mg

英文名字

MAL-dPEG??-TFP ester-1000 mg

供應(yīng)商

Click Chemistry Tools

產(chǎn)品貨號(hào)

CCT-QBD-10552-1000

產(chǎn)品報(bào)價(jià)

￥詢價(jià)/1000mg

產(chǎn)品說(shuō)明書(shū)

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購(gòu)買(mǎi)方式

銀行轉(zhuǎn)賬、電匯、支票、現(xiàn)金，在線支付寶及網(wǎng)銀支付，或直接與我們電話聯(lián)系。本公司所有產(chǎn)品僅供科研使用，不用于臨床診斷。

產(chǎn)品新聞

背景資料

MAL-dPEG?8-TFP酯(產(chǎn)品編號(hào)QBD-10552)是一種交聯(lián)劑，可通過(guò)中等長(zhǎng)度(38個(gè)原子)、單分子量、離散PEG(dPEG?)接頭將游離胺連接至游離巰基(巰基)。巰基通過(guò)邁克爾加成反應(yīng)與馬來(lái)酰亞胺基反應(yīng)。胺通過(guò)末端丙酸基團(tuán)的2，3，5，6-四氟苯基(TFP)酯的親核取代與交聯(lián)劑形成酰胺鍵。

產(chǎn)品描述

MAL-dPEG?8-TFP ester, product number QBD-10552, is a crosslinking reagent that joins free amines to free thiols (sulfhydryl groups) across a medium-length (38 atoms), single molecular weight, discrete PEG (dPEG?) linker. The sulfhydryl groups react with a maleimido group via a Michael addition reaction. The amines form amide bonds with the crosslinker by nucleophilic substitution of the 2,3,5,6-tetrafluorophenyl (TFP) ester of the terminal propionic acid group.nThe conjugation of free amines to free thiols is one of the most popular, most useful crosslinking reactions in bioconjugate chemistry is the conjugation of free amines to free thiols. These reactions require heterobifunctional reagents that bridge the two groups. Typical crosslinkers are hydrophobic. Vector Laboratories' dPEG? crosslinking products are water-soluble, amphiphilic, single molecular weight PEG compounds with discrete chain lengths. These hydrophilic, non-immunogenic, dPEG? crosslinkers prevent the problems with aggregation that arise when using traditional, hydrophobic crosslinkers.nPublished studies have shown that TFP esters are more hydrolytically stable in aqueous buffers than N-hydroxysuccinimidyl (NHS) esters and are more reactive toward free amines. TFP esters react under the same conditions as NHS esters. However, the optimal pH range for TFP esters (7.5 – 8.0) is slightly higher than for NHS esters (7.0 – 7.5). Amide bond formation between the TFP-activated propionic acid group of MAL-dPEG?8-TFP ester and a free amine will be slightly slower at a suboptimal pH compared to the reaction rate within the optimum pH range.nThe maleimide end of MAL-dPEG?8-TFP ester, product number QBD-10552, reacts optimally with a sulfhydryl at pH 6.5 – 7.5. Conduct the conjugation at the lowest reasonable pH within this range. Above pH 7.5, free amines compete with free thiols at the maleimide reaction site, which may confound the data. Moreover, at higher pH values, the maleimide ring may open to form unreactive maleamic acid.nMAL-dPEG?8-TFP ester, product number QBD-10552, can be used the same way and in the same applications as the equivalent NHS esters. Possible uses for this product include the following:nCoating nanoparticle surfaces;nTethering antibodies to atomic force microscopy (AFM) probes;nConstructing antibody-drug conjugates (ADCs);nAdding flexibility and water solubility to a peptide;nIncreasing the water solubility and hydrodynamic volume of hydrophobic biomolecules;nBuilding supramolecular constructs; and,nConjugating the TFP ester end of the molecule a liposomal surface and then using the free maleimide end of the molecule to attach a small molecule drug, peptide, or antibody for targeted delivery of a diagnostic or therapeutic package.

產(chǎn)品形式

無(wú)色至琥珀色粘稠液體;在儲(chǔ)存條件下可能固化為蠟狀固體

保存建議

其他

Click Chemistry Tools（CCT）公司位于美國(guó)亞利桑那州斯科茨代爾（Scottsdale,AZ）。自公司成立以來(lái)，一致致力于不斷開(kāi)發(fā)和擴(kuò)展一系列連接產(chǎn)品。他們利用特別新的研究成果研發(fā)生產(chǎn)出新穎的交聯(lián)和標(biāo)記試劑。產(chǎn)品被廣泛用于藥物研發(fā)、癌癥治療以及各類診斷技術(shù)等。此外，CCT還可以提供個(gè)性化的定制產(chǎn)品，已滿足不同用戶的需求。目前CCT的產(chǎn)品線主要包括：Azides（Biotin-Azides）試劑、Bis-sulfone交聯(lián)試劑、DBCO試劑、TCO試劑、Terminal Alkyne試劑、Tetrazine試劑、標(biāo)記試劑盒以及分選用底物等。nFluoroprobes公司位于美國(guó)亞利桑那州斯科茨代爾(Scottsdale, AZ)，自成立以來(lái)，一直致力于不斷開(kāi)發(fā)一系列的連接(linker)產(chǎn)品。利用特別新的研究成果研發(fā)生產(chǎn)出各類新穎的交聯(lián)和標(biāo)記試劑。其產(chǎn)品被廣泛用于藥物研發(fā)與篩選、疾病治療方法和診斷技術(shù)等領(lǐng)域。此外，F(xiàn)luoroprobes還可以根據(jù)客戶的個(gè)性化需求提供各類定制連接產(chǎn)品。代表產(chǎn)品有Classic Fluorescent Dyes, AZDye, Cyanine Dyes, IR Dyes, MB dyes以及一些標(biāo)記試劑盒等。nQuanta Biodesign公司位于美國(guó)中部的俄亥俄州，成立于1997年。也是專注于生產(chǎn)研發(fā)各類標(biāo)記產(chǎn)品的品牌之一。其研發(fā)的產(chǎn)品與dPEG技術(shù)相結(jié)合，生產(chǎn)出他們獨(dú)有的專利產(chǎn)品dPEG Building Blocks and Linkers，被廣泛用于細(xì)胞治療和分子診斷等研究領(lǐng)域。另外，還可為廣大科研工作者提供多種化學(xué)修飾試劑以及傳統(tǒng)Liner等。n目前上述三個(gè)品牌都已被被Vectorlabs收購(gòu)并整合到Vectorlabs產(chǎn)品中

注意

該頁(yè)面的中文產(chǎn)品信息的翻譯，僅供參考。準(zhǔn)確的產(chǎn)品信息請(qǐng)以廠家的英文說(shuō)明書(shū)為準(zhǔn)。下單前，請(qǐng)瀏覽說(shuō)明書(shū)確認(rèn)。

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