MitoPT? TMRM線粒體去極化分析試劑盒-MitoPT? TMRM Mitochondrial Depolarization Assay Kit代理/參數(shù)/廠家-細(xì)胞分析試劑盒-艾美捷科技有限公司

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背景資料: MitoPT TMRM檢測利用熒光染料TMRM檢測線粒體膜去極化。當(dāng)在負(fù)電荷極化的線粒體中積累時(shí)，TMRM發(fā)出橙色熒光。當(dāng)?shù)蛲黾?xì)胞或代謝應(yīng)激細(xì)胞的線粒體膜電位崩潰時(shí)，TMRM試劑在細(xì)胞胞漿中分散，熒光水平急劇下降。使用流式細(xì)胞儀、熒光板讀取器或熒光顯微鏡分析結(jié)果。

產(chǎn)品描述: 線粒體膜電位的喪失是細(xì)胞凋亡的標(biāo)志。ICT公司的MitoPT TMRM檢測技術(shù)利用電位熒光染料TMRM檢測線粒體膜通透性和膜去極化。TMRM染料具有分散在其分子結(jié)構(gòu)中的離域正電荷。此外，它的親脂性使它很容易被膜滲透并穿透活細(xì)胞。TMRM染料進(jìn)入帶負(fù)電荷的線粒體，在那里聚集并在興奮時(shí)發(fā)出橙色熒光。當(dāng)?shù)蛲黾?xì)胞的線粒體膜電位崩潰時(shí)，MitoPT TMRM分布在整個(gè)細(xì)胞質(zhì)中。檢測TMRM染色細(xì)胞中橙紅色熒光的丟失是評估實(shí)驗(yàn)細(xì)胞群中凋亡誘導(dǎo)或氧化應(yīng)激誘導(dǎo)線粒體去極化的可靠方法。

產(chǎn)品特點(diǎn): Loss of mitochondrial membrane potential is indicative of apoptosis. ICT’s MitoPT TMRM Assay utilizes the potentiometric fluorescent dye TMRM to detect mitochondrial membrane permeability and membrane depolarization. The TMRM dye has a delocalized positive charge dispersed throughout its molecular structure. In addition, its lipophilic solubility enables it to be readily membrane permeant and penetrate living cells. The TMRM dye enters the negatively charge mitochondria where it accumulates and fluoresces orange upon excitation. When the mitochondrial membrane potential collapses in apoptotic cells, MitoPT TMRM becomes distributed throughout the cytosol. Detection of the loss of orange-red fluorescence in TMRM stained cells is a reliable method for assessing apoptosis induction or oxidative stress-induced mitochondrial depolarization in experimental cell populations.

其他: ImmunoChemistry Technologies簡稱"ICT",成立于1994年,作為研發(fā)、銷售于一體的免疫化學(xué)生物技術(shù)公司,可以提供各種獨(dú)特的細(xì)胞蛋白酶、細(xì)胞凋亡與細(xì)胞毒性檢測試劑盒，同時(shí)提供全面的高質(zhì)量高靈敏度的 ELISA開發(fā)試劑，包括包被液、封閉液、沖洗液、底物、終止液、板子等等。 ??