NIR-FLIVO? 690 Tracer In vivo Assay-NIR-FLIVO? 690 Tracer In vivo Assay代理/參數(shù)/廠家-細(xì)胞分析試劑盒-艾美捷科技有限公司

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背景資料: FLIVO?(熒光活體)是一種強(qiáng)大的無(wú)創(chuàng)檢測(cè)體內(nèi)半胱天冬酶活性的方法。NIR-FLIVO 690示蹤劑是caspases的非細(xì)胞毒性、細(xì)胞透過(guò)熒光抑制劑，優(yōu)化用于整個(gè)活體動(dòng)物。ICT的NIR-FLIVO 690示蹤探針包含大多數(shù)caspases (Val-Ala-Asp或VAD)的首選結(jié)合序列。這個(gè)首選的聚半胱天冬酶三肽結(jié)合序列用*Dylight?690染料和氟甲基酮(FMK)反應(yīng)實(shí)體標(biāo)記。FLIVO?是ICT的注冊(cè)商標(biāo)。*Dylight?是Thermo Fisher Scientific, Inc.及其子公司的注冊(cè)商標(biāo)。

產(chǎn)品描述: FLIVO?試劑盒提供了一種簡(jiǎn)單、準(zhǔn)確的方法來(lái)檢測(cè)體內(nèi)caspase活性。像我們的FLICA?探針，但優(yōu)化了整個(gè)活的動(dòng)物標(biāo)記。為標(biāo)記含有活性caspases的細(xì)胞，靜脈注射FLIVO并讓其循環(huán)約60分鐘。由于試劑是細(xì)胞本身的，當(dāng)它在體內(nèi)循環(huán)時(shí)，它很容易在遇到的所有細(xì)胞內(nèi)擴(kuò)散或擴(kuò)散。如果細(xì)胞內(nèi)存在活躍的caspase酶，F(xiàn)LIVO會(huì)與caspase活性位點(diǎn)形成不可逆共價(jià)鍵。如果細(xì)胞膜完好，結(jié)合的FLIVO探針將留在細(xì)胞內(nèi)。任何未綁定的FLIVO將在大約一個(gè)小時(shí)內(nèi)從動(dòng)物的循環(huán)中移除。組織中剩余的近紅外熒光信號(hào)是注射試劑時(shí)發(fā)生的半胱天冬酶活性的直接測(cè)量。一旦多余的FLIVO從動(dòng)物體內(nèi)清除，動(dòng)物就可以進(jìn)行分析了。無(wú)需進(jìn)一步染色。使用整個(gè)活體動(dòng)物成像儀可以觀察到凋亡細(xì)胞和組織。或者，目標(biāo)組織可以被移除并進(jìn)行分析。FLIVO非常敏感，會(huì)檢測(cè)到自然發(fā)生的背景細(xì)胞凋亡。凋亡細(xì)胞的caspase活性高于對(duì)照細(xì)胞，因此它們?cè)贔LIVO作用下熒光更亮。

產(chǎn)品特點(diǎn): FLIVO? kits provide a simple and accurate method to detect caspase activity in vivo. Like our FLICA? probes but optimized for whole live animal labeling. To label cells containing active caspases, inject FLIVO intravenously and let it circulate ~60 minutes. Because the reagent is cell-permeant, it readily diffuses in and out of all cells that it encounters as it circulates throughout the body. If there are active caspase enzymes inside a cell, FLIVO will form an irreversible covalent bond with the caspase active site. The bound FLIVO probe will remain inside the cell if the cell membrane is intact. Any unbound FLIVO is removed from the circulation of the animal in about an hour. The remaining near-infrared fluorescent signal in the tissue is a direct measure of caspase activity that occurred at the time the reagent was injected. Once the excess FLIVO has cleared from the body of the animal, the animal is ready for analysis. No further staining is necessary. Apoptotic cells and tissues can be viewed using whole live animal imagers. Alternatively, target tissues can be removed and processed for analysis. FLIVO is very sensitive and will pick up naturally occurring background apoptosis. Apoptotic cells have more active caspases than control cells, therefore they fluoresce brighter with FLIVO.

其他: ImmunoChemistry Technologies簡(jiǎn)稱"ICT",成立于1994年,作為研發(fā)、銷售于一體的免疫化學(xué)生物技術(shù)公司,可以提供各種獨(dú)特的細(xì)胞蛋白酶、細(xì)胞凋亡與細(xì)胞毒性檢測(cè)試劑盒，同時(shí)提供全面的高質(zhì)量高靈敏度的 ELISA開發(fā)試劑，包括包被液、封閉液、沖洗液、底物、終止液、板子等等。 ??