HT-29裂解物通過在改良的RIPA緩沖液(150 mM氯化鈉,50 mM Tris-HCl,pH 7.4,ImM乙二胺四乙酸,ImM苯甲基磺酰氟,1%Triton X-100,1%脫氧膽酸鈉,0.1%十二烷基硫酸鈉,μ5g/ml抑肽酶,5gμ/ml亮抑酶肽)中均質(zhì)化制備。通過離心除去細(xì)胞碎片。用Bio-Rad蛋白質(zhì)測定法測定蛋白質(zhì)濃度。將HT-29裂解物在含有50 mM DTT的lx SDS樣品緩沖液(50 mM Tris-HCl pH 6.8,12.5%甘油,1%十二烷基硫酸鈉,0.01%溴酚藍(lán))中煮沸5分鐘。
產(chǎn)品描述
HT-29裂解物在含有50 mM DTT的SDS樣品緩沖液中提供。
產(chǎn)品形式
該HT-29 Lysate產(chǎn)品,產(chǎn)品形式為液體
保存建議
ProSci品牌廠家建議該HT-29 Lysate產(chǎn)品,Store at 2-8˚C for continuous use. For extended storage, freeze working aliquots at -70˚C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt.