通過(guò)在裂解緩沖液(IOmM HEPES pH7.9、1.5mM MgCl2、IOmM KCl、ImM乙二胺四乙酸、10%甘油、1%NP-40和蛋白酶抑制劑的混合物)中均質(zhì)化來(lái)制備前列腺組織裂解物。通過(guò)離心除去組織和細(xì)胞碎片。用Bio-Rad蛋白質(zhì)測(cè)定法測(cè)定蛋白質(zhì)濃度。將產(chǎn)物在含有50 mM DTT的lx SDS樣品緩沖液(50 mM Tris-HCl pH 6.8,12.5%甘油,1%十二烷基硫酸鈉,0.01%溴酚藍(lán))中煮沸5分鐘。
產(chǎn)品描述
前列腺裂解物在含有50mM DTT的SDS樣品緩沖液中提供。
產(chǎn)品形式
該P(yáng)rostate Lysate 產(chǎn)品,產(chǎn)品形式為液體
保存建議
ProSci品牌廠家建議該P(yáng)rostate Lysate 產(chǎn)品,Store at 2-8˚C for continuous use. For extended storage, freeze working aliquots at -70˚C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt.