外周血白細(xì)胞(PBL)組織裂解物通過在裂解緩沖液(10 mM HEPES pH7.9、1.5mM MgCl2、10 mM KCl、1 mM乙二胺四乙酸、10%甘油、1%NP-40和蛋白酶抑制劑的混合物)中均質(zhì)化來制備。通過離心除去組織和細(xì)胞碎片。將產(chǎn)物在含有50 mM DTT的lx SDS樣品緩沖液(50 mM Tris-HCl pH 6.8,12.5%甘油,1%十二烷基硫酸鈉,0.01%溴酚藍(lán))中煮沸5分鐘。
ProSci品牌廠家建議該P(yáng)eripheral Blood Leukocyte (PBL's) Lysate產(chǎn)品,Store at 2-8˚C for continuous use. For extended storage, freeze working aliquots at -70˚C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt.