通過在改良的RIPA緩沖液(150 mM氯化鈉、50 mM Tris-HCl,pH 7.4、ImM乙二胺四乙酸、ImM苯甲基磺酰氟、1%Triton X-100、1%脫氧膽酸鈉、0.1%十二烷基硫酸鈉、5%十二烷基硫酸鈉)中均質(zhì)化來制備胎兒裂解物(14天胎兒)。μg/ml抑肽酶,5μg/ml亮抑酶肽。通過離心除去組織和細(xì)胞碎片。用Bio-Rad蛋白質(zhì)測(cè)定法測(cè)定蛋白質(zhì)濃度。將產(chǎn)物在含有50 mM DTT的lx SDS樣品緩沖液(50 mM Tris-HCl pH 6.8,12.5%甘油,1%十二烷基硫酸鈉,0.01%溴酚藍(lán))中煮沸5分鐘。
產(chǎn)品描述
胎仔裂解物(14天胎仔)以含有50mM DTT的SDS樣品緩沖液提供。
產(chǎn)品形式
該Fetus Lysate (14 Day Fetus)產(chǎn)品,產(chǎn)品形式為液體
保存建議
ProSci品牌廠家建議該Fetus Lysate (14 Day Fetus)產(chǎn)品,Store at 2-8˚C for continuous use. For extended storage, freeze working aliquots at -70˚C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt.