Measure real-time cAMP signaling kinetics in the nucleus with this live cell fluorescent assay. Add NLS-tagged cADDis to your cells and measure robust signals the next day on a fluorescence imaging system. Decreasing green fluorescence indicates cAMP increase. This assay is genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the cAMP sensor BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (B2AR BacMam and 10mM isoproterenol in HCl). 10mL size is available as well. Other Montana Molecular tools including BacMam-packaged GPCRs, red fluorescent membrane-targeted cAMP sensors, or red fluorescent sensors for DAG or Calcium can easily be co-expressed with this assay.
產(chǎn)品描述
用這種活細(xì)胞熒光測(cè)定法測(cè)量細(xì)胞核中的實(shí)時(shí)cAMP信號(hào)傳導(dǎo)動(dòng)力學(xué)。將NLS標(biāo)記的cADD添加到您的細(xì)胞中,并在第二天在熒光成像系統(tǒng)上測(cè)量強(qiáng)大的信號(hào)。減少的綠色熒光指示cAMP增加。該檢測(cè)試劑在BacMam中進(jìn)行遺傳編碼和包裝,可在大多數(shù)細(xì)胞類(lèi)型中進(jìn)行有效轉(zhuǎn)導(dǎo)-包括在HEK 293細(xì)胞中的均勻和接近100%的效率以及在許多原代或iPSC衍生細(xì)胞中的表達(dá)。該試劑盒包括cAMP傳感器BacMam、500 mM丁酸鈉水溶液以及陽(yáng)性對(duì)照受體和激動(dòng)劑(B2 AR BacMam和10 mM異丙腎上腺素鹽酸溶液)。10 mL大小也是可用的。其他Montana分子工具,包括BacMam包裝的GPCR、紅色熒光膜靶向cAMP傳感器或DAG或鈣的紅色熒光傳感器,可以很容易地與該測(cè)定共表達(dá)。