Measure diacylglycerol (DAG) signaling kinetics, screen GPCRs for Gq activity, and more with this live cell fluorescent assay. Add the DAG sensor to your cells and measure robust signals after 30 hours on an automated plate reader or fluorescence imaging system. Decreasing red fluorescence indicates DAG increase. These assays are genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the DAG sensor BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (hM1R BacMam and 25mM carbachol in H2O). 10mL and 30mL sizes are available as well. Other Montana Molecular tools including BacMam-packaged GPCRs and green fluorescent cAMP, PIP2, or Calcium sensors can easily be co-expressed with this assay.
產(chǎn)品描述
測量二酰基甘油(DAG)信號動力學(xué),篩選Gq活性的GPCR,以及更多的活細(xì)胞熒光檢測。將DAG傳感器添加到細(xì)胞中,30小時后在自動酶標(biāo)儀或熒光成像系統(tǒng)上測量穩(wěn)健的信號。減少的紅色熒光指示DAG增加。這些檢測試劑在BacMam中進(jìn)行基因編碼和包裝,可在大多數(shù)細(xì)胞類型中進(jìn)行有效轉(zhuǎn)導(dǎo)-包括在HEK 293細(xì)胞中的均勻和接近100%的效率,以及在許多原代或iPSC衍生細(xì)胞中的表達(dá)。該試劑盒包括DAG傳感器BacMam、500 mM丁酸鈉水溶液以及陽性對照受體和激動劑(hM 1 R BacMam和25 mM卡巴膽堿水溶液)。10 mL和30 mL的尺寸也可用。其他Montana分子工具,包括BacMam包裝的GPCR和綠色熒光cAMP、PIP 2或鈣傳感器,可以很容易地與該測定共表達(dá)。