Measure arrestin signaling kinetics in real time with the live cell, fluorescent Borealis arrestin - Angiotensin II Type I receptor (AT1R) assay. Add the unmodified AT1R and Borealis sensor to cells and measure robust signals the next day on an automated plate reader or fluorescence imaging system. This assay is genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the arrestin sensor BacMam, AT1R BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (AT1R BacMam and 5mM Angiotensin II in H2O). 10mL and 30mL sizes are available as well, as are Borealis kits for several other receptors. Other Montana Molecular tools including the DAG and Calcium sensors can be used alongside this assay.
產品描述
用活細胞熒光Borealis arrestin-Angiotensin II Type I receptor(AT 1 R)測定法真實的實時測量arrestin信號傳導動力學。 將未修飾的AT 1 R和Borealis傳感器添加到細胞中,并在第二天在自動平板閱讀器或熒光成像系統(tǒng)上測量穩(wěn)健的信號。該檢測試劑在BacMam中進行遺傳編碼和包裝,可在大多數細胞類型中進行有效轉導-包括在HEK 293細胞中的均勻和接近100%的效率以及在許多原代或iPSC衍生細胞中的表達。該試劑盒包括抑制蛋白傳感器BacMam、AT 1 R BacMam、500 mM丁酸鈉水溶液以及陽性對照受體和激動劑(AT 1 R BacMam和5 mM血管緊張素II水溶液)。10 mL和30 mL規(guī)格也可供選擇,其他幾種受體的Borealis試劑盒也可供選擇。其他Montana Molecular工具,包括DAG和鈣傳感器,可與該測定一起使用。