Measure arrestin signaling kinetics in real time with the live cell, fluorescent Borealis arrestin - D1 Dopaine Receptor (D1R) assay. Add the unmodified D1 receptor and Borealis sensor to cells and measure robust signals the next day on an automated plate reader or fluorescence imaging system. This assay is genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the arrestin sensor BacMam, D1R BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (AT1R BacMam and 5mM Angiotensin II in H2O). 30mL and 60mL sizes are available as well, as are Borealis kits for several other receptors. Other Montana Molecular tools including the cADDis cAMP sensor can be used alongside this assay.
產(chǎn)品描述
用活細(xì)胞熒光Borealis arrestin-D1多巴胺受體(D1 R)測(cè)定法真實(shí)的實(shí)時(shí)測(cè)量arrestin信號(hào)傳導(dǎo)動(dòng)力學(xué)。 將未修飾的D1受體和Borealis傳感器添加到細(xì)胞中,并在第二天在自動(dòng)平板閱讀器或熒光成像系統(tǒng)上測(cè)量穩(wěn)健的信號(hào)。該檢測(cè)試劑在BacMam中進(jìn)行遺傳編碼和包裝,可在大多數(shù)細(xì)胞類(lèi)型中進(jìn)行有效轉(zhuǎn)導(dǎo)-包括在HEK 293細(xì)胞中的均勻和接近100%的效率以及在許多原代或iPSC衍生細(xì)胞中的表達(dá)。該試劑盒包括抑制蛋白傳感器BacMam、D1 R BacMam、500 mM丁酸鈉水溶液以及陽(yáng)性對(duì)照受體和激動(dòng)劑(AT 1 R BacMam和5 mM血管緊張素II水溶液)。30 mL和60 mL規(guī)格也可供選擇,其他幾種受體的Borealis試劑盒也可供選擇。其他Montana Molecular工具(包括cADDis cAMP傳感器)可與該測(cè)定一起使用。