Measure arrestin signaling kinetics in real time with the live cell, fluorescent Borealis arrestin - Oxytocin receptor (OTR) assay. Add the unmodified OTR and Borealis sensor to cells and measure robust signals the next day on an automated plate reader or fluorescence imaging system. This assay is genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the arrestin sensor BacMam, OTR BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (AT1R BacMam and 5mM Angiotensin II in H2O). 10mL and 60mL sizes are available as well, as are Borealis kits for several other receptors. Other Montana Molecular tools including the DAG and Calcium sensors can be used alongside this assay.
產(chǎn)品描述
用活細(xì)胞熒光Borealis抑制蛋白-催產(chǎn)素受體(OTR)測(cè)定法在真實(shí)的時(shí)間內(nèi)測(cè)量抑制蛋白信號(hào)傳導(dǎo)動(dòng)力學(xué)。 將未修飾的OTR和Borealis傳感器添加到細(xì)胞中,并在第二天在自動(dòng)化板讀取器或熒光成像系統(tǒng)上測(cè)量穩(wěn)健的信號(hào)。該檢測(cè)試劑在BacMam中進(jìn)行遺傳編碼和包裝,可在大多數(shù)細(xì)胞類型中進(jìn)行有效轉(zhuǎn)導(dǎo)-包括在HEK 293細(xì)胞中的均勻和接近100%的效率以及在許多原代或iPSC衍生細(xì)胞中的表達(dá)。該試劑盒包括抑制蛋白傳感器BacMam、OTR BacMam、500 mM丁酸鈉水溶液以及陽(yáng)性對(duì)照受體和激動(dòng)劑(AT 1 R BacMam和5 mM血管緊張素II水溶液)。還提供10毫升和60毫升規(guī)格,以及適用于其他幾種受體的Borealis試劑盒。其他Montana Molecular工具,包括DAG和鈣傳感器,可與該測(cè)定一起使用。