Measure cyclic AMP (cAMP) signaling kinetics, screen GPCRs for Gs activity, and more with the live cell, fluorescent, cADDis cAMP assay. Add the cADDis sensor to your cells and measure robust signals the next day on an automated plate reader or fluorescence imaging system. Increasing green fluorescence indicates cAMP increase. This assay is genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the cAMP sensor BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (B2AR BacMam and 10mM isoproterenol in HCl). 30mL and 60mL sizes are available as well. Other Montana Molecular tools including BacMam-packaged GPCRs or red fluorescent DAG and Calcium sensors can easily be co-expressed with this assay.
產(chǎn)品描述
測量環(huán)AMP(cAMP)信號傳導(dǎo)動力學(xué),篩選Gs活性的GPCR,以及使用活細(xì)胞、熒光、cADDis cAMP測定的更多功能。將cADDis傳感器添加到您的細(xì)胞中,并在第二天在自動酶標(biāo)儀或熒光成像系統(tǒng)上測量穩(wěn)健的信號。增加的綠色熒光指示cAMP增加。該檢測試劑在BacMam中進(jìn)行遺傳編碼和包裝,可在大多數(shù)細(xì)胞類型中進(jìn)行有效轉(zhuǎn)導(dǎo)-包括在HEK 293細(xì)胞中的均勻和接近100%的效率以及在許多原代或iPSC衍生細(xì)胞中的表達(dá)。該試劑盒包括cAMP傳感器BacMam、500 mM丁酸鈉水溶液以及陽性對照受體和激動劑(B2 AR BacMam和10 mM異丙腎上腺素HCl溶液)。30 mL和60 mL的尺寸也可用。其他Montana分子工具,包括BacMam包裝的GPCR或紅色熒光DAG和鈣傳感器,可以很容易地與該測定共表達(dá)。