Measure real-time cAMP signaling kinetics in the nucleus with this live cell fluorescent assay. Add NLS-tagged cADDis to your cells and measure robust signals the next day on a fluorescence imaging system. Increasing red fluorescence indicates cAMP increase. This assay is genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the cAMP sensor BacMam, 500mM sodium butyrate in H2O, and a positive control receptor and agonist (B2AR BacMam and 10mM isoproterenol in HCl). 10mL size is available as well. Other Montana Molecular tools including BacMam-packaged GPCRs, green fluorescent membrane-targeted or cytosolic cAMP sensors, or green fluorescent sensors for DAG or Calcium can easily be co-expressed with this assay.
產(chǎn)品描述
用這種活細胞熒光測定法測量細胞核中的實時cAMP信號傳導動力學。將NLS標記的cADD添加到您的細胞中,并在第二天在熒光成像系統(tǒng)上測量強大的信號。增加的紅色熒光指示cAMP增加。該檢測試劑在BacMam中進行遺傳編碼和包裝,可在大多數(shù)細胞類型中進行有效轉(zhuǎn)導-包括在HEK 293細胞中的均勻和接近100%的效率以及在許多原代或iPSC衍生細胞中的表達。該試劑盒包括cAMP傳感器BacMam、500 mM丁酸鈉水溶液以及陽性對照受體和激動劑(B2 AR BacMam和10 mM異丙腎上腺素HCl溶液)。10 mL大小也是可用的。其他Montana Molecular工具,包括BacMam包裝的GPCR、綠色熒光膜靶向或胞質(zhì)cAMP傳感器或用于DAG或鈣的綠色熒光傳感器,可以容易地與該測定共表達。